BRENDA - Enzyme Database
show all sequences of 1.3.5.5

Bleaching herbicide norflurazon inhibits phytoene desaturase by competition with the cofactors

Breitenbach, J.; Zhu, C.; Sandmann, G.; J. Agric. Food Chem. 49, 5270-5272 (2001) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Synechococcus sp.
expression in Escherichia coli
Gentiana lutea
Inhibitors
Inhibitors
Commentary
Organism
Structure
norflurazon
0.0001 mM, Vmax values are very little influenced by norflurazon, which reveals a competition between NADP+ or plastoquinone and norflurazon for the cofactor binding site of phytoene desaturase
Synechococcus sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
15-cis-phytoene + 2 plastoquinone
Synechococcus sp.
-
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
?
15-cis-phytoene + 2 plastoquinone
Gentiana lutea
-
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Gentiana lutea
-
-
-
Synechococcus sp.
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
15-cis-phytoene + 2 decyl-plastoquinone
this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially
712210
Synechococcus sp.
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
-
-
?
15-cis-phytoene + 2 decyl-plastoquinone
this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially. Decyl-plastoquinone is very effective as a cofactor of desaturation. The simultanous presence of NADP+ has no influence on the activity
712210
Gentiana lutea
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
-
-
?
15-cis-phytoene + 2 decyl-ubiquinone
this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially
712210
Synechococcus sp.
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
-
-
?
15-cis-phytoene + 2 decylubiquinone
this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially
712210
Gentiana lutea
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
-
-
?
15-cis-phytoene + 2 plastoquinone
-
712210
Synechococcus sp.
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
-
?
15-cis-phytoene + 2 plastoquinone
-
712210
Gentiana lutea
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
-
?
additional information
no activity with NADP+
712210
Gentiana lutea
?
-
-
-
?
additional information
the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone
712210
Synechococcus sp.
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
Synechococcus sp.
28
-
assay at
Gentiana lutea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Synechococcus sp.
7.2
-
assay at
Gentiana lutea
Cofactor
Cofactor
Commentary
Organism
Structure
decyl-plastoquinone
this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially
Gentiana lutea
additional information
the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone
Synechococcus sp.
plastoquinone
decyl-plastoquinone or decyl-ubiquinone is used in the study because the natural C45-plastoquinone is not available commercially. The affinity of the enzyme for plastoquinone is higher than for NADP+
Synechococcus sp.
Ubiquinone-10
this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially
Gentiana lutea
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Synechococcus sp.
expression in Escherichia coli
Gentiana lutea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
decyl-plastoquinone
this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially
Gentiana lutea
additional information
the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone
Synechococcus sp.
plastoquinone
decyl-plastoquinone or decyl-ubiquinone is used in the study because the natural C45-plastoquinone is not available commercially. The affinity of the enzyme for plastoquinone is higher than for NADP+
Synechococcus sp.
Ubiquinone-10
this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially
Gentiana lutea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
norflurazon
0.0001 mM, Vmax values are very little influenced by norflurazon, which reveals a competition between NADP+ or plastoquinone and norflurazon for the cofactor binding site of phytoene desaturase
Synechococcus sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
15-cis-phytoene + 2 plastoquinone
Synechococcus sp.
-
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
?
15-cis-phytoene + 2 plastoquinone
Gentiana lutea
-
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
15-cis-phytoene + 2 decyl-plastoquinone
this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially
712210
Synechococcus sp.
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
-
-
?
15-cis-phytoene + 2 decyl-plastoquinone
this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially. Decyl-plastoquinone is very effective as a cofactor of desaturation. The simultanous presence of NADP+ has no influence on the activity
712210
Gentiana lutea
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
-
-
?
15-cis-phytoene + 2 decyl-ubiquinone
this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially
712210
Synechococcus sp.
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
-
-
?
15-cis-phytoene + 2 decylubiquinone
this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially
712210
Gentiana lutea
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
-
-
?
15-cis-phytoene + 2 plastoquinone
-
712210
Synechococcus sp.
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
-
?
15-cis-phytoene + 2 plastoquinone
-
712210
Gentiana lutea
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
-
?
additional information
no activity with NADP+
712210
Gentiana lutea
?
-
-
-
?
additional information
the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone
712210
Synechococcus sp.
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
Synechococcus sp.
28
-
assay at
Gentiana lutea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Synechococcus sp.
7.2
-
assay at
Gentiana lutea
Other publictions for EC 1.3.5.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
743898
Sandmann
Control of light-dependent ke ...
Nostoc sp. PCC 7120 = FACHB-418
Z. Naturforsch. C
71
303-311
2016
-
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1
1
1
1
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-
743580
Gemmecker
Phytoene desaturase from Oryz ...
Oryza sativa Indica Group
PLoS ONE
10
e0131717
2015
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-
1
1
-
-
1
-
1
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1
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1
1
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1
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2
1
1
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1
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1
1
1
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1
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1
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1
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1
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1
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2
1
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742721
Alferez
Modification of carotenoid le ...
Citrus sinensis, Citrus sinensis L. Osbeck
J. Agric. Food Chem.
61
3082-3089
2013
-
1
1
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1
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1
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743489
Fantini
Dissection of tomato lycopene ...
Solanum lycopersicum
Plant Physiol.
163
986-998
2013
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1
1
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712989
Cong
cDNA cloning and expression an ...
Triticum aestivum
Mol. Biol. Rep.
37
3351-3361
2010
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5
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1
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713219
Lopez
Phytoene desaturase is present ...
Brassica oleracea
Physiol. Plant.
133
190-198
2008
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1
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3
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3
1
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9
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1
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5
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1
1
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1
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3
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3
1
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1
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5
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2
1
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711700
Qin
Disruption of phytoene desatur ...
Arabidopsis thaliana
Cell Res.
17
471-482
2007
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1
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1
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710925
Steinbrenner
Transformation of the green al ...
Haematococcus lacustris
Appl. Environ. Microbiol.
72
7477-7484
2006
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1
1
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1
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713245
Arias
Characterization of a higher p ...
Hydrilla verticillata
Plant Biotechnol. J.
4
263-273
2006
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660319
Breitenbach
zeta-Carotene cis isomers as p ...
Capsicum annuum
Planta
220
785-793
2005
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712212
Zhu
Isolation and characterization ...
Dunaliella salina
J. Agric. Food Chem.
53
5593-5597
2005
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659604
Matthews
Maize phytoene desaturase and ...
Zea mays
J. Exp. Bot.
54
2215-2230
2003
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712211
Ohki
Phytoene desaturase inhibition ...
Synechococcus elongatus PCC 7942 = FACHB-805
J. Agric. Food Chem.
51
3049-3055
2003
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10
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1
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10
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713208
Bennoun
The present model for chlorore ...
Embryophyta, Rhodophyta
Photosynth. Res.
73
273-277
2002
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2
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711506
Kita
Cloning and expression of CitP ...
Citrus unshiu
Biosci. Biotechnol. Biochem.
65
1424-1428
2001
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712209
Sandmann
In vitro inhibition studies of ...
Synechococcus sp.
J. Agric. Food Chem.
49
138-141
2001
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712210
Breitenbach
Bleaching herbicide norflurazo ...
Synechococcus sp., Gentiana lutea
J. Agric. Food Chem.
49
5270-5272
2001
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2
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1
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2
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8
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4
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4
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8
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713304
Grünewald
Phytoene desaturase is localiz ...
Haematococcus lacustris
Plant Physiol.
122
1261-1268
2000
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1
1
1
1
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713292
Wetzel
Regulation of phytoene desatur ...
Arabidopsis thaliana
Plant Mol. Biol.
37
1045-1053
1998
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1
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1
1
1
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713288
Al-Babili
A novel, soluble form of phyto ...
Narcissus pseudonarcissus
Plant J.
9
601-612
1996
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1
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1
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1
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713291
Li
Cloning and characterization o ...
Zea mays
Plant Mol. Biol.
30
269-279
1996
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1
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711883
Nievelstein
Carotene desaturation is linke ...
Narcissus pseudonarcissus
Eur. J. Biochem.
233
864-872
1995
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713273
Norris
Genetic dissection of caroteno ...
Arabidopsis thaliana
Plant Cell
7
2139-2149
1995
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1
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7910
Mayer
Quinone compounds are able to ...
Narcissus pseudonarcissus
Eur. J. Biochem.
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1990
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1
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