BRENDA - Enzyme Database show
show all sequences of 1.3.5.4

Plasticity of the quinone-binding site of the complex II homolog quinolfumarate reductase

Singh, P.K.; Sarwar, M.; Maklashina, E.; Kotlyar, V.; Rajagukguk, S.; Tomasiak, T.M.; Cecchini, G.; Iverson, T.M.; J. Biol. Chem. 288, 24293-24301 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Escherichia coli
Crystallization (Commentary)
Crystallization
Organism
subunit FrdC mutant E29L, to 2.95 A resolution. The sequential removal of the two menaquinol protons may be accompanied by a rotation of the naphthoquinone ring to optimize the interaction with a second proton shuttling pathway
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
E29L
mutation in subunit FrdC, alters hydrogen bonding to menaquinone
Escherichia coli
R28E/E29I
mutation in subunit FrdC, retains normal level of activity
Escherichia coli
R28L
mutation in subunit FrdC, retains normal level of activity
Escherichia coli
R28L/E9L
mutation in subunit FrdC, retains normal level of activity
Escherichia coli
R28L/R81A
mutation in subunits FrdC/FrdD, loss of activtiy with ubiquinone and menaquinol
Escherichia coli
R28N
mutation in subunit FrdC, retains normal level of activity
Escherichia coli
R81A
mutation in subunit FrdD, retains normal level of activity
Escherichia coli
R81E
mutation in subunit FrdD, retains normal level of activity
Escherichia coli
R81K
mutation in subunit FrdD, retains normal level of activity
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
-
-
menaquinol
mutant R28L, pH 7.0, 30°C
Escherichia coli
0.0005
-
ubiquinone
mutant R81E, pH 8.0, 30°C
Escherichia coli
0.0006
-
ubiquinone
mutant R81A, pH 8.0, 30°C
Escherichia coli
0.0009
-
menaquinol
mutant R81A, pH 7.0, 30°C
Escherichia coli
0.0013
-
menaquinol
mutant R81E, pH 7.0, 30°C
Escherichia coli
0.0013
-
ubiquinone
mutant R81K, pH 8.0, 30°C
Escherichia coli
0.0015
-
ubiquinone
mutant R28L, pH 8.0, 30°C; mutant R28N, pH 8.0, 30°C
Escherichia coli
0.002
-
ubiquinone
mutant R28L/E29L, pH 8.0, 30°C
Escherichia coli
0.0023
-
ubiquinone
wild-type, pH 8.0, 30°C
Escherichia coli
0.0027
-
menaquinol
mutant R81K, pH 7.0, 30°C
Escherichia coli
0.0031
-
menaquinol
mutant R28N, pH 7.0, 30°C
Escherichia coli
0.0038
-
ubiquinone
mutant R28E/E29I, pH 8.0, 30°C
Escherichia coli
0.0039
-
menaquinol
mutant R28L/E29L, pH 7.0, 30°C
Escherichia coli
0.004
-
menaquinol
mutant R28E/E29I, pH 7.0, 30°C
Escherichia coli
0.0054
-
menaquinol
wild-type, pH 7.0, 30°C
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P00363 and P0A8Q0 and P0A8Q3
P00363 i.e. subunit FrdA, P0A8Q0 i.e. subunit FrdC, P0A8Q3 i.e. subunit FrdD
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fumarate + menaquinol
-
742846
Escherichia coli
succinate + menaquinone
-
-
-
?
succinate + ferricyanide
-
742846
Escherichia coli
fumarate + ferrocyanide
-
-
-
?
succinate + ubiquinone
-
742846
Escherichia coli
fumarate + ubiquinol
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.7
-
ubiquinone
mutant R28E/E29I, pH 8.0, 30°C
Escherichia coli
2.8
-
menaquinol
mutant R28E/E29I, pH 8.0, 30°C
Escherichia coli
3
-
menaquinol
mutant R28E/E29I, pH 7.0, 30°C
Escherichia coli
6.1
-
menaquinol
mutant R81A, pH 8.0, 30°C
Escherichia coli
10.6
-
menaquinol
mutant R81A, pH 7.0, 30°C
Escherichia coli
10.7
-
ubiquinone
mutant R81E, pH 8.0, 30°C
Escherichia coli
11.1
-
menaquinol
mutant R28L/E29L, pH 8.0, 30°C
Escherichia coli
12.2
-
menaquinol
mutant R28L/E29L, pH 7.0, 30°C
Escherichia coli
12.8
-
menaquinol
mutant R81E, pH 8.0, 30°C
Escherichia coli
13.9
-
ubiquinone
mutant R28L/E29L, pH 8.0, 30°C
Escherichia coli
14
-
menaquinol
mutant R81E, pH 7.0, 30°C
Escherichia coli
17.4
-
ubiquinone
mutant R81A, pH 8.0, 30°C
Escherichia coli
19
-
ubiquinone
mutant R28N, pH 8.0, 30°C
Escherichia coli
19.3
-
ubiquinone
mutant R28L, pH 8.0, 30°C
Escherichia coli
20.1
-
ferricyanide
mutant R28L/R81A, pH 8.0, 30°C
Escherichia coli
24.6
-
ferricyanide
mutant R28N, pH 8.0, 30°C
Escherichia coli
24.9
-
ubiquinone
wild-type, pH 8.0, 30°C
Escherichia coli
26.4
-
ferricyanide
mutant R28E/E29I, pH 8.0, 30°C
Escherichia coli
27.1
-
ferricyanide
mutant R28L, pH 8.0, 30°C
Escherichia coli
27.3
-
ferricyanide
mutant R81E, pH 8.0, 30°C
Escherichia coli
28.1
-
ferricyanide
mutant R81A, pH 8.0, 30°C
Escherichia coli
28.7
-
ferricyanide
wild-type, pH 8.0, 30°C
Escherichia coli
28.9
-
ferricyanide
mutant R28L/E29L, pH 8.0, 30°C
Escherichia coli
30.4
-
ferricyanide
mutant R81K, pH 8.0, 30°C
Escherichia coli
32.2
-
ubiquinone
mutant R81K, pH 8.0, 30°C
Escherichia coli
65.4
-
menaquinol
mutant R28N, pH 8.0, 30°C
Escherichia coli
67.8
-
menaquinol
mutant R28L, pH 8.0, 30°C
Escherichia coli
68.6
-
menaquinol
mutant R28N, pH 7.0, 30°C
Escherichia coli
71.1
-
menaquinol
mutant R28L, pH 7.0, 30°C
Escherichia coli
123.7
-
menaquinol
mutant R81K, pH 7.0, 30°C
Escherichia coli
138.9
-
menaquinol
mutant R81K, pH 8.0, 30°C
Escherichia coli
211
-
menaquinol
wild-type, pH 8.0, 30°C
Escherichia coli
222.2
-
menaquinol
wild-type, pH 7.0, 30°C
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Escherichia coli
[3Fe-4S]-center
located in subunit FrdC
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Escherichia coli
[3Fe-4S]-center
located in subunit FrdC
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
subunit FrdC mutant E29L, to 2.95 A resolution. The sequential removal of the two menaquinol protons may be accompanied by a rotation of the naphthoquinone ring to optimize the interaction with a second proton shuttling pathway
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E29L
mutation in subunit FrdC, alters hydrogen bonding to menaquinone
Escherichia coli
R28E/E29I
mutation in subunit FrdC, retains normal level of activity
Escherichia coli
R28L
mutation in subunit FrdC, retains normal level of activity
Escherichia coli
R28L/E9L
mutation in subunit FrdC, retains normal level of activity
Escherichia coli
R28L/R81A
mutation in subunits FrdC/FrdD, loss of activtiy with ubiquinone and menaquinol
Escherichia coli
R28N
mutation in subunit FrdC, retains normal level of activity
Escherichia coli
R81A
mutation in subunit FrdD, retains normal level of activity
Escherichia coli
R81E
mutation in subunit FrdD, retains normal level of activity
Escherichia coli
R81K
mutation in subunit FrdD, retains normal level of activity
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
-
-
menaquinol
mutant R28L, pH 7.0, 30°C
Escherichia coli
0.0005
-
ubiquinone
mutant R81E, pH 8.0, 30°C
Escherichia coli
0.0006
-
ubiquinone
mutant R81A, pH 8.0, 30°C
Escherichia coli
0.0009
-
menaquinol
mutant R81A, pH 7.0, 30°C
Escherichia coli
0.0013
-
menaquinol
mutant R81E, pH 7.0, 30°C
Escherichia coli
0.0013
-
ubiquinone
mutant R81K, pH 8.0, 30°C
Escherichia coli
0.0015
-
ubiquinone
mutant R28L, pH 8.0, 30°C; mutant R28N, pH 8.0, 30°C
Escherichia coli
0.002
-
ubiquinone
mutant R28L/E29L, pH 8.0, 30°C
Escherichia coli
0.0023
-
ubiquinone
wild-type, pH 8.0, 30°C
Escherichia coli
0.0027
-
menaquinol
mutant R81K, pH 7.0, 30°C
Escherichia coli
0.0031
-
menaquinol
mutant R28N, pH 7.0, 30°C
Escherichia coli
0.0038
-
ubiquinone
mutant R28E/E29I, pH 8.0, 30°C
Escherichia coli
0.0039
-
menaquinol
mutant R28L/E29L, pH 7.0, 30°C
Escherichia coli
0.004
-
menaquinol
mutant R28E/E29I, pH 7.0, 30°C
Escherichia coli
0.0054
-
menaquinol
wild-type, pH 7.0, 30°C
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fumarate + menaquinol
-
742846
Escherichia coli
succinate + menaquinone
-
-
-
?
succinate + ferricyanide
-
742846
Escherichia coli
fumarate + ferrocyanide
-
-
-
?
succinate + ubiquinone
-
742846
Escherichia coli
fumarate + ubiquinol
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.7
-
ubiquinone
mutant R28E/E29I, pH 8.0, 30°C
Escherichia coli
2.8
-
menaquinol
mutant R28E/E29I, pH 8.0, 30°C
Escherichia coli
3
-
menaquinol
mutant R28E/E29I, pH 7.0, 30°C
Escherichia coli
6.1
-
menaquinol
mutant R81A, pH 8.0, 30°C
Escherichia coli
10.6
-
menaquinol
mutant R81A, pH 7.0, 30°C
Escherichia coli
10.7
-
ubiquinone
mutant R81E, pH 8.0, 30°C
Escherichia coli
11.1
-
menaquinol
mutant R28L/E29L, pH 8.0, 30°C
Escherichia coli
12.2
-
menaquinol
mutant R28L/E29L, pH 7.0, 30°C
Escherichia coli
12.8
-
menaquinol
mutant R81E, pH 8.0, 30°C
Escherichia coli
13.9
-
ubiquinone
mutant R28L/E29L, pH 8.0, 30°C
Escherichia coli
14
-
menaquinol
mutant R81E, pH 7.0, 30°C
Escherichia coli
17.4
-
ubiquinone
mutant R81A, pH 8.0, 30°C
Escherichia coli
19
-
ubiquinone
mutant R28N, pH 8.0, 30°C
Escherichia coli
19.3
-
ubiquinone
mutant R28L, pH 8.0, 30°C
Escherichia coli
20.1
-
ferricyanide
mutant R28L/R81A, pH 8.0, 30°C
Escherichia coli
24.6
-
ferricyanide
mutant R28N, pH 8.0, 30°C
Escherichia coli
24.9
-
ubiquinone
wild-type, pH 8.0, 30°C
Escherichia coli
26.4
-
ferricyanide
mutant R28E/E29I, pH 8.0, 30°C
Escherichia coli
27.1
-
ferricyanide
mutant R28L, pH 8.0, 30°C
Escherichia coli
27.3
-
ferricyanide
mutant R81E, pH 8.0, 30°C
Escherichia coli
28.1
-
ferricyanide
mutant R81A, pH 8.0, 30°C
Escherichia coli
28.7
-
ferricyanide
wild-type, pH 8.0, 30°C
Escherichia coli
28.9
-
ferricyanide
mutant R28L/E29L, pH 8.0, 30°C
Escherichia coli
30.4
-
ferricyanide
mutant R81K, pH 8.0, 30°C
Escherichia coli
32.2
-
ubiquinone
mutant R81K, pH 8.0, 30°C
Escherichia coli
65.4
-
menaquinol
mutant R28N, pH 8.0, 30°C
Escherichia coli
67.8
-
menaquinol
mutant R28L, pH 8.0, 30°C
Escherichia coli
68.6
-
menaquinol
mutant R28N, pH 7.0, 30°C
Escherichia coli
71.1
-
menaquinol
mutant R28L, pH 7.0, 30°C
Escherichia coli
123.7
-
menaquinol
mutant R81K, pH 7.0, 30°C
Escherichia coli
138.9
-
menaquinol
mutant R81K, pH 8.0, 30°C
Escherichia coli
211
-
menaquinol
wild-type, pH 8.0, 30°C
Escherichia coli
222.2
-
menaquinol
wild-type, pH 7.0, 30°C
Escherichia coli
Other publictions for EC 1.3.5.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743255
Kassem
The impairment of methylmenaq ...
Campylobacter jejuni subsp. jejuni
MicrobiologyOpen
3
168-181
2014
-
-
-
-
-
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-
-
-
-
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-
-
1
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-
-
-
-
-
-
-
-
1
1
-
-
-
742846
Singh
Plasticity of the quinone-bin ...
Escherichia coli
J. Biol. Chem.
288
24293-24301
2013
-
-
1
1
9
-
-
15
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
33
-
-
-
2
-
-
-
-
-
1
2
1
9
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
33
-
-
-
-
-
-
-
-
-
-
743059
Nasiri
Design, synthesis, and biolog ...
Wolinella succinogenes, Wolinella succinogenes DSM 1740
J. Med. Chem.
56
9530-9541
2013
-
-
-
-
-
-
2
-
-
-
-
-
-
2
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-
-
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-
4
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4
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4
2
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724561
Herzog
Hydrogen-bonded networks along ...
Campylobacter jejuni, Helicobacter pylori, Wolinella succinogenes
Biophys. J.
103
1305-1314
2012
-
-
-
-
3
-
-
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
2
3
-
-
-
-
-
-
-
6
-
-
-
-
-
-
6
-
3
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
3
3
-
-
-
725316
Shimizu
Crystal structure of mitochond ...
Ascaris suum
J. Biochem.
151
589-592
2012
-
-
-
1
-
-
-
-
1
1
-
1
-
1
-
-
1
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1
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-
2
2
-
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3
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3
1
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1
1
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1
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-
1
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
700278
Juhnke
Production, characterization a ...
Campylobacter jejuni, Wolinella succinogenes
Mol. Microbiol.
71
1088-1101
2009
-
-
-
-
1
-
-
-
2
-
-
1
-
5
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
707545
Xin
Purification, characterization ...
Chloroflexus aurantiacus
Biochim. Biophys. Acta
1787
86-96
2009
-
-
-
1
-
-
1
-
1
1
4
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
1
-
-
-
1
-
-
1
1
4
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707949
Garcia
The succinate:menaquinone redu ...
Bacillus cereus
Can. J. Microbiol.
54
456-466
2008
-
-
-
-
-
-
2
2
1
-
-
-
-
3
-
-
-
1
-
-
1
-
2
1
1
-
-
1
1
-
-
2
1
-
-
-
-
-
2
-
-
-
-
2
1
2
1
-
-
-
-
-
-
-
-
-
1
-
2
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
674532
Maklashina
Fumarate reductase and succina ...
Escherichia coli
J. Biol. Chem.
281
11357-11365
2006
-
-
-
1
3
-
2
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
6
-
-
-
-
6
-
-
-
-
-
-
1
3
-
-
2
6
5
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