Application | Comment | Organism |
---|---|---|
synthesis | expression of full-length and N-terminally trucated enzyme lacking 29 amino acids in an Escherichia coli system | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | cardiolipin enhances the interaction of truncated DHODH with lipid bilayers, but the presence of the transmembrane domain in human DHODH is necessary for stable binding to and securing its location at the outer surface of the inner mitochondrial membrane | Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q02127 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using immobilized Ni2+ affinity chromatography, full-length protein is solubilized by dodecyl D-maltoside | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
solubilization of full-length enzyme using by dodecyl D-maltoside in order to obtain planar lipid bilayers that are detergent-free. The presence of tetraoleyl cardiolipin in the bilayers significantly enhances the binding between the bilayer and truncated human DHODH. Addition of coenzyme Q10 does not have a detectable effect when compared to bilayers consisting only of 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
69 | - |
recombinant full-length enzyme, pH 8.0, 30°C | Homo sapiens |
94 | - |
recombinant N-terminally truncated enzyme, pH 8, 30°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + decylubiquinone | - |
Homo sapiens | orotate + reduced decylubiquinone | - |
? |