N111A |
large decrease in reduction rate constant |
Escherichia coli |
N111D |
large decrease in reduction rate constant. Reduction potential is about 100 mV lower than in wild-type |
Escherichia coli |
N172A |
large decrease in reduction rate constant. Reduction potential is about 25 mV lower than in wild-type |
Escherichia coli |
N172A/N246A |
large decrease in reduction rate constant. The maximum flavin absorbance is at 453 nm, blue-shifted 3 nm compared to wild type |
Escherichia coli |
N177A |
large decrease in reduction rate constant. Reduction potential is about 25 mV lower than in wild-type |
Escherichia coli |
N246A |
large decrease in reduction rate constant |
Escherichia coli |