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Literature summary for 1.3.5.2 extracted from
- Purification, characterization and localization of mitochondrial dihydroorotate dehydrogenase in Plasmodium falciparum, human malaria parasite (1995), Biochim. Biophys. Acta, 1243, 351-360.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-aminoorotate | - |
Plasmodium falciparum |  |
| 5-bromoorotate | - |
Plasmodium falciparum | |
| 5-Fluoroorotate | - |
Plasmodium falciparum | |
| 5-iodoorotate | - |
Plasmodium falciparum | |
| 5-Methylorotate | - |
Plasmodium falciparum | |
| atovaquone | - |
Plasmodium falciparum | |
| benzoquinones | ubiquinone-0 and decylubiquinone | Plasmodium falciparum | |
| chloronaphthoquinone | - |
Plasmodium falciparum | |
| Lapachol | - |
Plasmodium falciparum | |
| Lawsone | - |
Plasmodium falciparum | |
| menadione | - |
Plasmodium falciparum | |
| Orotate | also inhibitors: 5-substituted orotates; competitive to dihydroorotate; strong competitive inhibitor | Plasmodium falciparum | |
| rhodamine 123 | mitochondrial inhibitor | Plasmodium falciparum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
dihydroorotate | - |
Plasmodium falciparum | |
| 0.0144 | - |
S-dihydroorotate | - |
Plasmodium falciparum | |
| 0.022 | - |
coenzyme Q6 | - |
Plasmodium falciparum | |
| 0.0225 | - |
ubiquinone-6 | - |
Plasmodium falciparum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Plasmodium falciparum | 5739 | - |
| mitochondrion | inner membrane | Plasmodium falciparum | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
gel filtration | Plasmodium falciparum |
| 56000 | - |
gel filtration, SDS-PAGE | Plasmodium falciparum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-dihydroorotate + ubiquinone | Plasmodium falciparum | fourth step in synthesis of uridine 5'-monophosphate | orotate + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
| Plasmodium falciparum | - |
mature trophozytes and schizonts | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Plasmodium falciparum |
| cation-, anion-exchange, gel filtration | Plasmodium falciparum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0002672 | - |
37°C | Plasmodium falciparum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, addition of S-dihydroorotate to stabilize the enzyme activity | Plasmodium falciparum |
| 4°C, extremely labile, 90% decrease of activity over night | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-dihydroorotate + ubiquinone | ubiquinone-6, ubiquinone-7, ubiquinone-9 and ubiquinone-10 maximize enzyme activity | Plasmodium falciparum | orotate + ubiquinol | - |
? | |
| (S)-dihydroorotate + ubiquinone | fourth step in synthesis of uridine 5'-monophosphate | Plasmodium falciparum | orotate + ubiquinol | - |
? | |
| dihydroorotate + acceptor | acceptor: quinones | Plasmodium falciparum | orotate + reduced acceptor | - |
? | |
| L-dihydroorotate + ubiquinone-6 | - |
Plasmodium falciparum | orotate + reduced ubiquinone-6 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 56000, SDS-PAGE | Plasmodium falciparum |
| monomer | SDS-PAGE | Plasmodium falciparum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
S-dihydroorotate | - |
Plasmodium falciparum | |
| 0.36 | - |
ubiquinone6 | - |
Plasmodium falciparum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Plasmodium falciparum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0151 | - |
5-Fluoroorotate | - |
Plasmodium falciparum | |
| 0.018 | - |
Orotate | - |
Plasmodium falciparum | |
| 0.0182 | - |
Orotate | - |
Plasmodium falciparum | |
| 0.0244 | - |
5-aminoorotate | - |
Plasmodium falciparum | |
| 0.1185 | - |
5-Methylorotate | - |
Plasmodium falciparum | |
| 0.1408 | - |
5-bromoorotate | - |
Plasmodium falciparum | |
| 0.2246 | - |
5-iodoorotate | - |
Plasmodium falciparum | |
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