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Literature summary for 1.3.5.2 extracted from

  • Palfey, B.A.; Bjoernberg, O.; Jensen, K.F.
    Insight into the chemistry of flavin reduction and oxidation in Escherichia coli dihydroorotate dehydrogenase obtained by rapid reaction studies (2001), Biochemistry, 40, 4381-4390.
    View publication on PubMed
Search for more literature for 1.3.5.2

Protein Variants

Protein Variants Comment Organism
S175A less than 0.01% of activity of the wild-type enzyme Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-dihydroorotate + acceptor Escherichia coli fourth step in synthesis of pyrimidine nucleotides orotate + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
 family 2 enzyme
-

Reaction

Reaction Comment Organism Reaction ID
(S)-dihydroorotate + a quinone = orotate + a quinol existence of 3 families differing in their selectivity for oxidizing substrates: 1A are soluble, containing FMN and use fumarate, 1B are soluble, one subunit contains an iron-sulfur center, FAD and reduces NAD+, family 2 enzymes are membrane-bound, contain FMN and are oxidized by ubiquinone. Mechanism consists of 3 reaction phases. Different binding-mechanisms for enzyme the reaction-steps are suggested Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + acceptor uses ubiquinone as electron acceptor Escherichia coli orotate + reduced acceptor
-
 ?
(S)-dihydroorotate + acceptor fourth step in synthesis of pyrimidine nucleotides Escherichia coli orotate + reduced acceptor
-
 ?
(S)-dihydroorotate + ubiquinone reaction studied with menadione, O2 and ferricyanide Escherichia coli orotate + ubiquinol
-
 ?
dihydroorotate + acceptor reaction intermediates, mechanism, pH-dependence of reaction Escherichia coli orotate + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
DHOD
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.7
-
 S-dihydroorotate pH 8.5, 4°C, with menadione Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 12.7
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Escherichia coli