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Literature summary for 1.3.5.1 extracted from
- Atypical features of Thermus thermophilus succinate:quinone reductase (2013), PLoS ONE, 8, e53559.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of C-terminally His6-tagged or N-termminally His8-tagged enzyme in Thermus thermophilus under the control of a constitutive promoter | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-nitropropionic acid | - |
Thermus thermophilus |  |
| malonate | - |
Thermus thermophilus | |
| nonyl-4-hydroxyquinoline-N-oxide | the semiquinone analog and inhibitor of quinone reactions in complex II shows no influence on the redox behavior of the heme b moieties | Thermus thermophilus | |
| oxaloacetate | - |
Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | interprotomer temperature-dependent positive cooperativity in the trimeric complex. Only the trimer, not the monomer, exhibits positive cooperativity at high temperatures | Thermus thermophilus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Thermus thermophilus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the enzyme contains a [3Fe-4S] center | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 119800 | - |
monomeric recombinant C-terminally His6-tagged enzyme, gel filtration | Thermus thermophilus |
| 120000 | - |
1 * 120000, about, recombinant C-terminally His6-tagged enzyme, SDS-PAGE, trimerization is disrupted in rcIISdhB-His6 due to the insertion of a hexahistidine tag on the C-terminus of SdhB subunit and the resulting protein complex can only form a monomer | Thermus thermophilus |
| 120000 | - |
3 * 120000, about, wild-type enzyme, SDS-PAGE | Thermus thermophilus |
| 120000 | - |
4 * 120000, about, recombinant N-terminally His8-tagged enzyme, SDS-PAGE, four subunits of the rcII-His8-SdhB complex | Thermus thermophilus |
| 500000 | - |
wild-type enzyme, consisting of 360 kDa from protein and an unknown contribution of detergent and lipid, native PAGE | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate + menaquinone | Thermus thermophilus | - |
fumarate + menaquinol | - |
? | |
| succinate + menaquinone | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
fumarate + menaquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6- or His8-tagged enzyme from Thermus thermophilus by nickel affinity chromatography and gel filtration, untagged enzyme by anion exchange chromatography and gel filtration | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinate + menaquinone | - |
Thermus thermophilus | fumarate + menaquinol | - |
? | |
| succinate + menaquinone | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | fumarate + menaquinol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 120000, about, recombinant C-terminally His6-tagged enzyme, SDS-PAGE, trimerization is disrupted in rcIISdhB-His6 due to the insertion of a hexahistidine tag on the C-terminus of SdhB subunit and the resulting protein complex can only form a monomer | Thermus thermophilus |
| tetramer | 4 * 120000, about, recombinant N-terminally His8-tagged enzyme, SDS-PAGE, four subunits of the rcII-His8-SdhB complex | Thermus thermophilus |
| trimer | 3 * 120000, about, wild-type enzyme, SDS-PAGE | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SQR | - |
Thermus thermophilus |
| succinate:quinone reductase | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Thermus thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | covalently-bound flavin cofactor | Thermus thermophilus | |
| heme | the enzyme contains two heme b cofactors, a di-heme | Thermus thermophilus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | succinate:quinone reductase serves as the respiratory complex II | Thermus thermophilus |
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