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Literature summary for 1.3.3.8 extracted from
- Pathway engineering of benzylisoquinoline alkaloid biosynthesis in transgenic California poppy cells with ectopic expression of tetrahydroberberine oxidase from Coptis japonica (2012), Plant Biotechnol., 29, 473-481.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, functional ectopical expression of CjTHBO under the control of Cauliflower mosaic virus 35S promoterin Eschscholzia californica cells via transformation with Agrobacterium tumefaciens strain LBA4404. The transgenic cells produce coptisine and dehydrocheilanthifoline, which are expected to be generated by the reaction of CjTHBO from pathway intermediates, i.e. cheilanthifoline and stylopine, alkaloid profile analysis in transgenic California poppy cells, overview. Expression in Escherichia coli strain BL21 (DE3) | Coptis japonica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57748 | - |
x * 57748, sequence calculation | Coptis japonica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-tetrahydroberberine + 2 O2 | Coptis japonica | - |
berberine + 2 H2O2 | - |
? |  |
| additional information | Coptis japonica | the recombinant enzyme expressed in transgenic Eschscholzia californica cells produces coptisine and dehydrocheilanthifoline from pathway intermediates cheilanthifoline and stylopine, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coptis japonica | E3WDH1 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Coptis japonica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-tetrahydroberberine + 2 O2 | - |
Coptis japonica | berberine + 2 H2O2 | - |
? | |
| additional information | the recombinant enzyme expressed in transgenic Eschscholzia californica cells produces coptisine and dehydrocheilanthifoline from pathway intermediates cheilanthifoline and stylopine, overview | Coptis japonica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57748, sequence calculation | Coptis japonica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (S)-tetrahydroberberine oxidase | - |
Coptis japonica |
| THBO | - |
Coptis japonica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Coptis japonica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Coptis japonica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Coptis japonica | sequence calculation | - |
6.1 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | (S)-tetrahydroberberine oxidase is the enzyme in the last step of berberine biosynthesis | Coptis japonica |
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Release 2023.1 (January 2023)
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