Cloned (Comment) | Organism |
---|---|
- |
Coptis japonica |
General Stability | Organism |
---|---|
instable | Coptis japonica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bathophenanthroline sulfonic acid | specific Fe2+ ion chelator, more than 1 mM required for 50% inhibition at an enzyme concentration of 0.0003 mM | Coptis japonica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
(S)-tetrahydroberberine | - |
Coptis japonica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Coptis japonica |
58000 | - |
gel filtration | Coptis japonica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-tetrahydroberberine + 2 O2 | Coptis japonica | final step in berberine biosynthesis | berberine + 2 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coptis japonica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ion-exchange, gel filtration | Coptis japonica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Coptis japonica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
8.1 nkat/mg protein | Coptis japonica |
Storage Stability | Organism |
---|---|
4°C, 50% loss of activity after 20 h | Coptis japonica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-tetrahydroberberine + 2 O2 | four hydrogen atoms from the substrate are transferred to two molecules of oxygen to produce two molecules of hydrogen peroxide and one molecule of berberine | Coptis japonica | berberine + 2 H2O2 | - |
? | |
(S)-tetrahydroberberine + 2 O2 | final step in berberine biosynthesis | Coptis japonica | berberine + 2 H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE | Coptis japonica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Coptis japonica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
50% loss of activity after 20 h | Coptis japonica |
45 | - |
1 h, 40% loss of activity | Coptis japonica |
50 | - |
1 h, 90% loss of activity | Coptis japonica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
50 mM phosphate buffer and borate buffer | Coptis japonica |