Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Bromouracil | competitive vs. dihydrouracil | Rhodotorula glutinis | |
5-Chlorouracil | competitive vs. dihydrouracil | Rhodotorula glutinis | |
5-fluorouracil | competitive vs. dihydrouracil | Rhodotorula glutinis | |
5-Iodouracil | competitive vs. dihydrouracil | Rhodotorula glutinis | |
5-Nitrouracil | competitive vs. dihydrouracil | Rhodotorula glutinis | |
p-chloromercuribenzoate | - |
Rhodotorula glutinis | |
thymine | competitive vs. dihydrouracil | Rhodotorula glutinis | |
Uracil | competitive vs. dihydrouracil | Rhodotorula glutinis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
dihydrouracil | - |
Rhodotorula glutinis | |
0.05 | - |
Dihydrothymine | - |
Rhodotorula glutinis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Rhodotorula glutinis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula glutinis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex a-50, electrofocusing, Sephadex G-200, partially purified | Rhodotorula glutinis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.25 | - |
- |
Rhodotorula glutinis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dihydrouracil + O2 | O2 is the only known electron acceptor, no substrates are dihydro-6-methyluracil, dihydro D-orotic acid and dihydro L-orotic acid | Rhodotorula glutinis | uracil + H2O2 | - |
? | |
dihydrothymine + O2 | - |
Rhodotorula glutinis | thymine + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Rhodotorula glutinis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable up to | Rhodotorula glutinis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Rhodotorula glutinis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | 1 h, 25°C, stable | Rhodotorula glutinis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | flavoprotein | Rhodotorula glutinis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
thymine | - |
Rhodotorula glutinis | |
0.0035 | - |
5-Nitrouracil | - |
Rhodotorula glutinis | |
0.01 | - |
5-fluorouracil | - |
Rhodotorula glutinis | |
0.02 | - |
5-Chlorouracil | - |
Rhodotorula glutinis | |
0.032 | - |
5-Bromouracil | - |
Rhodotorula glutinis | |
0.035 | - |
Uracil | - |
Rhodotorula glutinis | |
0.088 | - |
5-Iodouracil | - |
Rhodotorula glutinis |