Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain XL-1 Blue in inclusion bodies | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
Y366A | site-directed mutagenesis, the mutant enzyme shows 10% of wild-type enzyme activity | Bacillus subtilis |
Y366E | site-directed mutagenesis, the mutant enzyme shows 1% of wild-type enzyme activity | Bacillus subtilis |
Y366H | site-directed mutagenesis, the mutant enzyme shows 10% of wild-type enzyme activity | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acifluorfen | - |
Bacillus subtilis | |
additional information | Bacillus subtilis PPO is unique because of its resistance to inhibition by diphenylethers involving residue Y366 at a site adjacent to substrate and FAD cofactor, modeling and docking studies, overview | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00067 | - |
protoporphyrinogen IX | pH 7.5, 22°C, recombinant wild-type enzyme | Bacillus subtilis | |
0.00107 | - |
protoporphyrinogen IX | pH 7.5, 22°C, recombinant mutant Y366A | Bacillus subtilis | |
0.00234 | - |
protoporphyrinogen IX | pH 7.5, 22°C, recombinant mutant Y366E | Bacillus subtilis | |
0.00354 | - |
protoporphyrinogen IX | pH 7.5, 22°C, recombinant mutant Y366H | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen IX + 3 O2 | Bacillus subtilis | - |
protoporphyrin + 3 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P32397 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain XL-1 Blue inclusion bodies | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen IX + 3 O2 | - |
Bacillus subtilis | protoporphyrin + 3 H2O2 | - |
? | |
protoporphyrinogen IX + 3 O2 | Bacillus subtilis PPO shows a broad substrate specificity involving residue Y366 at a site adjacent to substrate and FAD cofactor, modeling and docking studies and structure-function relationship, overview | Bacillus subtilis | protoporphyrin + 3 H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | modeling and docking studies and structure-function relationship, overview. Homology modelling and structure comparisons, overview | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
PPO | - |
Bacillus subtilis |
protoporphyrinogen IX oxidase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
protoporphyrinogen IX | pH 7.5, 22°C, recombinant mutant Y366E | Bacillus subtilis | |
0.00042 | - |
protoporphyrinogen IX | pH 7.5, 22°C, recombinant mutant Y366A | Bacillus subtilis | |
0.0016 | - |
protoporphyrinogen IX | pH 7.5, 22°C, recombinant mutant Y366H | Bacillus subtilis | |
0.0034 | - |
protoporphyrinogen IX | pH 7.5, 22°C, recombinant wild-type enzyme | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
acifluorfen | pH 7.5, 22°C, recombinant mutant Y366A | Bacillus subtilis | |
0.033 | - |
acifluorfen | pH 7.5, 22°C, recombinant wild-type enzyme | Bacillus subtilis | |
0.053 | - |
acifluorfen | pH 7.5, 22°C, recombinant mutant Y366H | Bacillus subtilis | |
0.154 | - |
acifluorfen | pH 7.5, 22°C, recombinant mutant Y366E | Bacillus subtilis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.133 | - |
pH 7.5, 22°C, recombinant mutant Y366H | Bacillus subtilis | acifluorfen | |
0.173 | - |
pH 7.5, 22°C, recombinant mutant Y366A | Bacillus subtilis | acifluorfen | |
0.183 | - |
pH 7.5, 22°C, recombinant wild-type enzyme | Bacillus subtilis | acifluorfen | |
0.617 | - |
pH 7.5, 22°C, recombinant mutant Y366E | Bacillus subtilis | acifluorfen |
General Information | Comment | Organism |
---|---|---|
metabolism | protoporphyrinogen IX oxidase is the last common enzyme of heme and chlorophyll biosynthesis | Bacillus subtilis |