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Literature summary for 1.3.3.4 extracted from

  • Watanabe, N.; Takayama, S.; Yoshida, S.; Isogai, A.; Che, F.S.
    Resistance to protoporphyrinogen oxidase-inhibiting compound S23142 from overproduction of mitochondrial protoporphyrinogen oxidase by gene amplification in photomixotrophic tobacco cells (2002), Biosci. Biotechnol. Biochem., 66, 1799-1805.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overproduction of mitochondrial isozyme by gene amplification in photomixotrophic cells Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
5-[2-chloro-4-(trifluoromethyl)phenoxyl]-2-nitrobenzoic acid transgenic YZI-1S cells show a 150fold greater resistance to the inhibitor compared to wild-type cells, inhibition of mitochondrial and chloroplastic isozymes Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast plastidic isozyme Nicotiana tabacum 9507
-
membrane both isozymes Nicotiana tabacum 16020
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mitochondrion mitochondrial isozyme Nicotiana tabacum 5739
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additional information activity in chloroplast of wild-type and transgenic cells is similar for both isozymes, while the transgenic mitochondria show 5.3fold higher activity than those from the wild-type Nicotiana tabacum
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrinogen-IX + 3 O2 Nicotiana tabacum
-
protoporphyrin-IX + 3 H2O2
-
?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
cv. Samsun NN, 2 isozymes in chloroplasts and mitochondria
-

Purification (Commentary)

Purification (Comment) Organism
both isozymes partially from cell culture of wild-type and transgenic cells, by chloroplast and mitochondria fractionation, detergent solubilization, and ultracentrifugation Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture photomixotrophic cells Nicotiana tabacum
-
YZI-1S cell transgenic cell with 150fold greater resistance to inhibitor S23142 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity in chloroplast of wild-type and transgenic cells is similar for both isozymes, while the transgenic mitochondria show 5.3fold higher activity than those from the wild-type Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrinogen-IX + 3 O2
-
Nicotiana tabacum protoporphyrin-IX + 3 H2O2
-
?

Synonyms

Synonyms Comment Organism
protox
-
Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Nicotiana tabacum