Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain JM109 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G11A | site-directed mutagenesis, 0.02% activity compared to the wild-type enzyme | Homo sapiens |
G14A | site-directed mutagenesis, 42.6% activity compared to the wild-type enzyme | Homo sapiens |
G9A | site-directed mutagenesis, 0.52% activity compared to the wild-type enzyme | Homo sapiens |
H20P | site-directed mutagenesis, 0.16% activity compared to the wild-type enzyme | Homo sapiens |
additional information | identification of several mutations of the enzyme encoding gene, responsible for variegate porphyria, overview | Homo sapiens |
R168C | site-directed mutagenesis, 17.5% activity compared to the wild-type enzyme | Homo sapiens |
R59I | site-directed mutagenesis, 1.5% activity compared to the wild-type enzyme | Homo sapiens |
R59K | site-directed mutagenesis, 37.6% activity compared to the wild-type enzyme | Homo sapiens |
R59S | site-directed mutagenesis, 2.6% activity compared to the wild-type enzyme | Homo sapiens |
R59W | site-directed mutagenesis, 0.28% activity compared to the wild-type enzyme, highly reduced FAD binding | Homo sapiens |
Y348C | site-directed mutagenesis, 8.6% activity compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acifluorfen | inhibition potency with recombinant wild-type and mutant enzymes, overview | Homo sapiens | |
bilirubin | inhibition potency with recombinant wild-type and mutant enzymes, overview | Homo sapiens | |
biliverdin IX hydrochloride | inhibition potency with recombinant wild-type and mutant enzymes, overview | Homo sapiens | |
methyl acifluorfen | inhibition potency with recombinant wild-type and mutant enzymes, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00083 | - |
protoporphyrinogen-IX | recombinant mutant R59K, pH 8.2 | Homo sapiens | |
0.00085 | - |
protoporphyrinogen-IX | recombinant wild-type enzyme and mutant G14A, pH 8.2 | Homo sapiens | |
0.001 | - |
protoporphyrinogen-IX | recombinant mutant R168C, pH 8.2 | Homo sapiens | |
0.00107 | - |
protoporphyrinogen-IX | recombinant mutant Y348, pH 7.8 | Homo sapiens | |
0.00126 | - |
protoporphyrinogen-IX | recombinant mutant R59W, pH 8.2 | Homo sapiens | |
0.0017 | - |
protoporphyrinogen-IX | recombinant mutant R59S, pH 8.2 | Homo sapiens | |
0.00209 | - |
protoporphyrinogen-IX | recombinant mutant R59I, pH 8.2 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Homo sapiens | 5743 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen-IX + 3 O2 | Homo sapiens | penultimate enzyme in the heme biosynthesis, several mutations of the enzyme encoding gene are responsible for variegate porphyria | protoporphyrin-IX + 3 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain JM109, to homogeneity | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
renaturation and refolding after thermal denaturation and unfolding at 75°C by cooling to 15°C, pH 7.2 | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
0.62 | - |
recombinant mutant Y348C | Homo sapiens |
1.26 | - |
recombinant mutant R168C | Homo sapiens |
2.69 | - |
recombinant mutant R59K | Homo sapiens |
3.04 | - |
recombinant mutant G14A | Homo sapiens |
7.15 | - |
recombinant wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen-IX + 3 O2 | penultimate enzyme in the heme biosynthesis, several mutations of the enzyme encoding gene are responsible for variegate porphyria | Homo sapiens | protoporphyrin-IX + 3 H2O2 | - |
? | |
protoporphyrinogen-IX + 3 O2 | roles of 59 arginine residues and glycine residues in the flavin binding site in catalysis and cofactor binding | Homo sapiens | protoporphyrin-IX + 3 H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000, SDS-PAGE | Homo sapiens |
More | secondary structure analysis, wild-type and mutant enzymes, structure/function relationship mutant R59W | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PPOX | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant wild-type and mutants, stable at | Homo sapiens |
47.6 | - |
50% inactivation of mutant R168C | Homo sapiens |
50.2 | - |
50% inactivation of mutant Y348C | Homo sapiens |
53 | - |
50% inactivation of mutant R59W | Homo sapiens |
54.2 | - |
50% inactivation of mutant R59I | Homo sapiens |
56.3 | - |
50% inactivation of mutant R59K | Homo sapiens |
56.8 | - |
50% inactivation of recombinant wild-type enzyme | Homo sapiens |
57 | - |
50% inactivation of mutant G14A | Homo sapiens |
60.3 | - |
50% inactivation of mutant R59S | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
protoporphyrinogen-IX | recombinant mutant R59W, pH 8.2 | Homo sapiens | |
0.15 | - |
protoporphyrinogen-IX | recombinant mutant R59I, pH 8.2 | Homo sapiens | |
0.17 | - |
protoporphyrinogen-IX | recombinant mutant R59S, pH 8.2 | Homo sapiens | |
0.53 | - |
protoporphyrinogen-IX | recombinant mutant Y348, pH 7.8 | Homo sapiens | |
1.02 | - |
protoporphyrinogen-IX | recombinant mutant R168C, pH 8.2 | Homo sapiens | |
2.57 | - |
protoporphyrinogen-IX | recombinant mutant R59K, pH 8.2 | Homo sapiens | |
3.9 | - |
protoporphyrinogen-IX | recombinant mutant G14A, pH 8.2 | Homo sapiens | |
5.95 | - |
protoporphyrinogen-IX | recombinant wild-type enzyme, pH 8.2 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
mutant Y348C | Homo sapiens |
8.2 | - |
except for mutant Y348C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required, binding site, structure, and mechanism, roles of 59 arginine residues and glycine residues in the flavin binding site in catalysis and cofactor binding | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00011 | - |
acifluorfen | recombinant mutant R59I, pH 8.2 | Homo sapiens | |
0.00018 | - |
acifluorfen | recombinant mutant R59K, pH 8.2 | Homo sapiens | |
0.00023 | - |
acifluorfen | recombinant wild-type, pH 8.2 | Homo sapiens | |
0.00027 | - |
acifluorfen | recombinant mutant R59S, pH 8.2 | Homo sapiens | |
0.00031 | - |
acifluorfen | recombinant mutant G14A, pH 8.2 | Homo sapiens | |
0.00033 | - |
acifluorfen | recombinant mutant R59W, pH 8.2 | Homo sapiens | |
0.003 | - |
acifluorfen | recombinant mutant R168C, pH 8.2 | Homo sapiens | |
0.00472 | - |
acifluorfen | recombinant mutant Y348C, pH 7.8 | Homo sapiens |