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Literature summary for 1.3.3.4 extracted from
- The enzymic conversion of protoporphyrinogen IX to protoporphyrin IX. Protoporphyrinogen oxidase activity in mitochondrial extracts of Saccharomyces cerevisiae (1975), J. Biol. Chem., 250, 1269-1274.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | - |
Saccharomyces cerevisiae |  |
| dithiothreitol | - |
Saccharomyces cerevisiae | |
| glutathione | - |
Saccharomyces cerevisiae | |
General Stability
| General Stability | Organism |
|---|---|
| sensitive towards trypsin and chymotrypsin | Saccharomyces cerevisiae |
| stable on dialysis | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | slight inhibition | Saccharomyces cerevisiae | |
| Co2+ | inhibitory | Saccharomyces cerevisiae | |
| Cu2+ | inhibitory | Saccharomyces cerevisiae | |
| heme | noncompetitive, reversible by dilution | Saccharomyces cerevisiae | |
| hemin | noncompetitive, reversible by dilution | Saccharomyces cerevisiae | |
| iodoacetamide | slight inhibition | Saccharomyces cerevisiae | |
| iodoacetate | slight inhibition | Saccharomyces cerevisiae | |
| additional information | inhibited by high salt concentrations | Saccharomyces cerevisiae | |
| p-hydroxymercuribenzoate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0048 | - |
protoporphyrinogen-IX | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrinogen-IX + 3 O2 | Saccharomyces cerevisiae | penultimate step of heme and chlorophyll biosynthesis | protoporphyrin-IX + 3 H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0026 | - |
purified enzyme | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 50 mM Tris-HCl in presence of 20% glycerol and 1 mM EDTA, several months, no loss of activity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mesoporphyrinogen-IX + 3 O2 | 20% of the rate of protoporphyrinogen-IX | Saccharomyces cerevisiae | mesoporphyrin-IX + 3 H2O2 | - |
? | |
| protoporphyrinogen-IX + 3 O2 | - |
Saccharomyces cerevisiae | protoporphyrin-IX + 3 H2O2 | - |
? | |
| protoporphyrinogen-IX + 3 O2 | penultimate step of heme and chlorophyll biosynthesis | Saccharomyces cerevisiae | protoporphyrin-IX + 3 H2O2 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
inactivated above 40°C | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | - |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9.5 | unstable at higher and lower pH | Saccharomyces cerevisiae |
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