Literature summary for 1.3.3.3 extracted from

Stephenson, J.R.; Stacey, J.A.; Morgenthaler, J.B.; Friesen, J.A.; Lash, T.D.; Jones, M.A.
Role of aspartate 400, arginine 262, and arginine 401 in the catalytic mechanism of human coproporphyrinogen oxidase (2007), Protein Sci., 16, 401-410 .

Search for more literature for 1.3.3.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D400A	mutation severely impaires the catalytic activity, mutant performs, on average, less than a single turnover	Homo sapiens
R262A	mutation results in decreased kcat values and increased Km values. No product formation is detected with sunstrate mesoporphyrogen-VI	Homo sapiens
R401A	mutation results in decreased kcat values and increased Km values. The mutant is able to convert mesoporphyrogen-VI to monovinyl product, although over 10fold less efficiently than wild-type	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00034	-	Coproporphyrinogen-III	wild-type, divinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.00056	-	mesoporphyrinogen-VI	wild-type, monovinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.00078	-	Coproporphyrinogen-III	mutant D400A, total product, 37°C, pH not specified in the publication	Homo sapiens
0.00079	-	Coproporphyrinogen-III	wild-type, total product, 37°C, pH not specified in the publication	Homo sapiens
0.00096	-	Coproporphyrinogen-III	mutant R401A, divinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.002	-	mesoporphyrinogen-VI	mutant R401A, monovinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.0029	-	Coproporphyrinogen-III	mutant R401A, total product, 37°C, pH not specified in the publication	Homo sapiens
0.0051	-	Coproporphyrinogen-III	mutant R262A, divinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.0087	-	Coproporphyrinogen-III	mutant R262A, total product, 37°C, pH not specified in the publication	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P36551	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, Ni-NTA agarose metal affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
coproporphyrinogen-III + O2 + 2 H+	-	Homo sapiens	protoporphyrinogen-IX + 2 CO2 + 2 H2O	-	?
harderoporphyrinogen + O2 + 2 H+	substrate undergoes only the second decarboxylation	Homo sapiens	protoporphyrinogen + CO2 + H2O	-	?
mesoporphyrinogen-VI + O2 + H+	substrate undergoes only the first oxidative decarboxylation	Homo sapiens	? + CO2 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CPOX	-	Homo sapiens
oxygen-dependent coproporphyrinogen-III oxidase	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000002	-	Coproporphyrinogen-III	mutant D400A, total product, 37°C, pH not specified in the publication	Homo sapiens
0.000012	-	Coproporphyrinogen-III	mutant R262A, divinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.00042	-	Coproporphyrinogen-III	mutant R262A, total product, 37°C, pH not specified in the publication	Homo sapiens
0.00167	-	Coproporphyrinogen-III	mutant R401A, divinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.002	-	mesoporphyrinogen-VI	mutant R401A, monovinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.0063	-	mesoporphyrinogen-VI	wild-type, monovinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.02	-	Coproporphyrinogen-III	mutant R401A, total product, 37°C, pH not specified in the publication	Homo sapiens
0.02667	-	Coproporphyrinogen-III	wild-type, divinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.045	-	Coproporphyrinogen-III	wild-type, total product, 37°C, pH not specified in the publication	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.000003	-	Coproporphyrinogen-III	mutant R262A, divinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.000027	-	Coproporphyrinogen-III	mutant D400A, total product, 37°C, pH not specified in the publication	Homo sapiens
0.000048	-	Coproporphyrinogen-III	mutant R262A, total product, 37°C, pH not specified in the publication	Homo sapiens
0.001	-	mesoporphyrinogen-VI	mutant R401A, monovinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.00183	-	Coproporphyrinogen-III	mutant R401A, divinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.00683	-	Coproporphyrinogen-III	mutant R401A, total product, 37°C, pH not specified in the publication	Homo sapiens
0.0113	-	mesoporphyrinogen-VI	wild-type, monovinyl product, 37°C, pH not specified in the publication	Homo sapiens
0.0567	-	Coproporphyrinogen-III	wild-type, total product, 37°C, pH not specified in the publication	Homo sapiens
0.07	-	Coproporphyrinogen-III	wild-type, divinyl product, 37°C, pH not specified in the publication	Homo sapiens

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Release 2023.1 (January 2023)