Cloned (Comment) | Organism |
---|---|
functional expression in Escherichia coli | Plasmodium falciparum |
recombinant expression of His-tagged enzyme in Escherichia coli | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00967 | - |
Coproporphyrinogen III | pH 7.5, 37°C, recombinant enzyme | Plasmodium falciparum | |
0.0151 | - |
Coproporphyrinogen III | pH 7.5, 37°C, native enzyme | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Plasmodium falciparum | 5829 | - |
cytosol | exclusively | Plasmodium falciparum | 5829 | - |
additional information | no localization in the mitochondrion | Plasmodium falciparum | - |
- |
additional information | PfCPO does not carry an obvious N-terminal leader targeting sequence, subcellular localization study, overview. No measurable activity in the solubilized parasite membrane fraction | Plasmodium falciparum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metal ions Cu2+, Mg2+, Mn2+, Fe2+ and Zn2+ at up to 0.500 mM concentration have no effect on CPO activity | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
1 * 58000, recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
61000 | - |
recombinant enzyme, gel filtration | Plasmodium falciparum |
61000 | - |
recombiinant enzyme, gel filtration | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + O2 + 2 H+ | Plasmodium falciparum | - |
protoporphyrinogen IX + 2 CO2 + 2 H2O | - |
? | |
coproporphyrinogen III + O2 + 2 H+ | Plasmodium falciparum | - |
protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli, cleavage of the tag | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + O2 + 2 H+ | - |
Plasmodium falciparum | protoporphyrinogen IX + 2 CO2 + 2 H2O | - |
? | |
coproporphyrinogen III + O2 + 2 H+ | - |
Plasmodium falciparum | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
additional information | no activity with S-adenosylmethionine or without O2 | Plasmodium falciparum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
More | sequence comparisons, overview | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
coproporphyrinogen III oxidase | - |
Plasmodium falciparum |
CPO | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Plasmodium falciparum |
37 | - |
recombinant enzyme | Plasmodium falciparum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | activity range, recombinant enzyme | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Plasmodium falciparum |
7.6 | - |
recombinant enzyme | Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | activity range, recombinant enzyme | Plasmodium falciparum |