Literature summary for 1.3.3.3 extracted from

Zeilstra-Ryalls, J.H.; Schornberg, K.L.
Analysis of hemF gene function and expression in Rhodobacter sphaeroides 2.4.1 (2006), J. Bacteriol., 188, 801-804.

Activating Compound

Activating Compound	Comment	Organism	Structure
oxygen	levels of hemF protein are much higher in cells grown aerobically than in anaerobically grown cells, amount correlates with gene dosage	Cereibacter sphaeroides

Cloned(Commentary)

Cloned (Comment)	Organism
into the MscI site of pUI1087, generating pKS7, moving of the hemF sequences from pKS7 into pBBR1MCS2, creating pKS9, BamHI DNA fragment deleted from pKS9, omega Sp-St resistance cassette inserted at the remaining unique BamHI site, generating pKS11, from pKS11 3135-bp SmaI fragment containing delta hemF::omegaSpr-Str moved into pSUP202, replacing the vector sequences contained on a 1,348-bp ScaI fragment and creating pKS13, hemF::lacZ transcription reporter plasmid pJZ84 constructed by first inserting a 717-bp SmaI-NcoI DNA fragment from pKS9 into pUI1087, using the flanking PstI-XbaI vector restriction sites, the hemFequences are positioned and correctly oriented in front of a promoterless lacZ gene in plasmid vector pCF1010	Cereibacter sphaeroides

Cloned (Comment)

Organism

into the MscI site of pUI1087, generating pKS7, moving of the hemF sequences from pKS7 into pBBR1MCS2, creating pKS9, BamHI DNA fragment deleted from pKS9, omega Sp-St resistance cassette inserted at the remaining unique BamHI site, generating pKS11, from pKS11 3135-bp SmaI fragment containing delta hemF::omegaSpr-Str moved into pSUP202, replacing the vector sequences contained on a 1,348-bp ScaI fragment and creating pKS13, hemF::lacZ transcription reporter plasmid pJZ84 constructed by first inserting a 717-bp SmaI-NcoI DNA fragment from pKS9 into pUI1087, using the flanking PstI-XbaI vector restriction sites, the hemFequences are positioned and correctly oriented in front of a promoterless lacZ gene in plasmid vector pCF1010

Cereibacter sphaeroides

Protein Variants

Protein Variants	Comment	Organism
additional information	HemF- mutant strain, unable to grow under aerobic condition	Cereibacter sphaeroides

Organism

Organism	UniProt	Comment	Textmining
Cereibacter sphaeroides	-	strain 2.4.1, mutant strain JZ3534	-

Synonyms

Synonyms	Comment	Organism
coproporphyrinogen III oxidase	-	Cereibacter sphaeroides
HemF	-	Cereibacter sphaeroides

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Release 2023.1 (January 2023)