Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
25 mg/ml purified recombinant His-tagged enzyme in 20 mM Tris, pH 7.5, 5% v/v glycerol, sitting drop method, 21°C, for C-form crystals: equal volume of protein and reservoir solution, the latter containing 20% PEG 3000, 0.1 M HEPES, pH 7.5, and 0.2 M sodium acetate, optimized sulfur anomalous scattering, for form 1 crystals: sitting drops of protein and reservoir solution, the latter containing 18% PEG 8000, 0.1 M HEPES, pH 7.5, 2% isopropanol, and 0.2 M sodium acetate at 4°C, 13°C, or 21°C, for form II crystals: sitting drops of protein and reservoir solution in a 2:1 mixture, the latter containing 2.2 M ammonium sulfate, 0.1 M Tris, pH 8.5, 21°C, 10% v/v glycerol as cryoprotectant, X-ray diffraction structure determination and anaylsis at 2.0 A and 2.4 A resolution | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | identification of diverse deleterious enzyme mutations in hereditary coproporphyria, structural effects of mutations, overview | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, recombinant enzyme, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen-III + O2 | Saccharomyces cerevisiae | essential enzyme, catalyzes the 6th step in heme biosynthesis | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
additional information | Saccharomyces cerevisiae | deleterious enzyme mutations can cause hereditary coproporphyria | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
coproporphyrinogen III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O | active site structure | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen-III + O2 | essential enzyme, catalyzes the 6th step in heme biosynthesis | Saccharomyces cerevisiae | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
coproporphyrinogen-III + O2 | O2-dependent enzyme Hem13p | Saccharomyces cerevisiae | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
? | |
additional information | deleterious enzyme mutations can cause hereditary coproporphyria | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, recombinant enzyme, SDS-PAGE | Saccharomyces cerevisiae |
More | central flat seven-stranded antiparallel sheet flanked by alpha-helices | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
CPO | - |
Saccharomyces cerevisiae |
Hem13p | - |
Saccharomyces cerevisiae |