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Literature summary for 1.3.3.17 extracted from

  • Schuehle, K.; Saft, M.; Voegeli, B.; Erb, T.J.; Heider, J.
    Benzylmalonyl-CoA dehydrogenase, an enzyme involved in bacterial auxin degradation (2021), Arch. Microbiol., 203, 4149-4159 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.3.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Aromatoleum aromaticum

Inhibitors

Inhibitors Comment Organism Structure
benzylmalonyl-CoA strong substrate inhibition Aromatoleum aromaticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 benzylmalonyl-CoA at pH 7.8, temperature not specified in the publication Aromatoleum aromaticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
159000
-
 gel filtration Aromatoleum aromaticum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzylmalonyl-CoA + O2 Aromatoleum aromaticum
-
 (E)-cinnamoyl-CoA + CO2 + H2O2
-
 ir
benzylmalonyl-CoA + O2 Aromatoleum aromaticum EbN1
-
 (E)-cinnamoyl-CoA + CO2 + H2O2
-
 ir

Organism

Organism UniProt Comment Textmining
Aromatoleum aromaticum
-
-
-
Aromatoleum aromaticum EbN1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-tag affinity column chromatography, and Superdex 200 gel filtration Aromatoleum aromaticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3-methyl)-butylmalonyl-CoA + O2 low specific activity Aromatoleum aromaticum ?
-
 ?
(3-methyl)-butylmalonyl-CoA + O2 low specific activity Aromatoleum aromaticum EbN1 ?
-
 ?
benzylmalonyl-CoA + O2
-
 Aromatoleum aromaticum (E)-cinnamoyl-CoA + CO2 + H2O2
-
 ir
benzylmalonyl-CoA + O2 high activity. The enzyme is enantiospecific in benzylmalonyl-CoA turnover Aromatoleum aromaticum (E)-cinnamoyl-CoA + CO2 + H2O2
-
 ir
benzylmalonyl-CoA + O2
-
 Aromatoleum aromaticum EbN1 (E)-cinnamoyl-CoA + CO2 + H2O2
-
 ir
benzylmalonyl-CoA + O2 high activity. The enzyme is enantiospecific in benzylmalonyl-CoA turnover Aromatoleum aromaticum EbN1 (E)-cinnamoyl-CoA + CO2 + H2O2
-
 ir
butylmalonyl-CoA + O2 low specific activity Aromatoleum aromaticum ?
-
 ?
butylmalonyl-CoA + O2 low specific activity Aromatoleum aromaticum EbN1 ?
-
 ?
hexylmalonyl-CoA + O2 low specific activity Aromatoleum aromaticum ?
-
 ?
additional information the enzyme is inert with ethylmalonyl-CoA, methylmalonyl-CoA and phenylpropionyl-CoA. The enzyme shows no activity with electron transfer flavoprotein or artificial dyes serving as electron acceptors like ferricenium cation or phenazine-methosulfate/dichlorophenyl-indophenol. The enzyme also does not convert cinnamoyl-CoA to either benzylmalonyl-CoA (with carboxylation) or phenylpropionyl-CoA (without carboxylation) by using dithionite-reduced benzyl or methyl viologen Aromatoleum aromaticum ?
-
-
additional information the enzyme is inert with ethylmalonyl-CoA, methylmalonyl-CoA and phenylpropionyl-CoA. The enzyme shows no activity with electron transfer flavoprotein or artificial dyes serving as electron acceptors like ferricenium cation or phenazine-methosulfate/dichlorophenyl-indophenol. The enzyme also does not convert cinnamoyl-CoA to either benzylmalonyl-CoA (with carboxylation) or phenylpropionyl-CoA (without carboxylation) by using dithionite-reduced benzyl or methyl viologen Aromatoleum aromaticum EbN1 ?
-
-

Subunits

Subunits Comment Organism
homotetramer 4 * 41000, SDS-PAGE Aromatoleum aromaticum

Synonyms

Synonyms Comment Organism
iaaF
-
 Aromatoleum aromaticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5
-
 benzylmalonyl-CoA at pH 7.8, temperature not specified in the publication Aromatoleum aromaticum

Cofactor

Cofactor Comment Organism Structure
FAD the FAD content of the enzyme is 4.2 per homotetramer Aromatoleum aromaticum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0091
-
 benzylmalonyl-CoA at pH 7.8, temperature not specified in the publication Aromatoleum aromaticum