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Literature summary for 1.3.3.15 extracted from

  • Yoon, J.; Han, Y.; Ahn, Y.O.; Hong, M.K.; Sung, S.K.
    Characterization of HemY-type protoporphyrinogen IX oxidase genes from cyanobacteria and their functioning in transgenic Arabidopsis (2019), Plant Mol. Biol., 101, 561-574 .
    View publication on PubMed
Search for more literature for 1.3.3.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Arabidopsis thaliana and Escherichia coli strain BT3 Oscillatoria nigro-viridis
expressed in Arabidopsis thaliana and Escherichia coli strain BT3 Halothece sp. PCC7418

Inhibitors

Inhibitors Comment Organism Structure
saflufenacil
-
 Halothece sp. PCC7418
saflufenacil
-
 Oscillatoria nigro-viridis
tiafenacil
-
 Halothece sp. PCC7418
tiafenacil
-
 Oscillatoria nigro-viridis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0077
-
 protoporphyrinogen IX pH and temperature not specified in the publication Halothece sp. PCC7418
0.0091
-
 protoporphyrinogen IX pH and temperature not specified in the publication Oscillatoria nigro-viridis

Organism

Organism UniProt Comment Textmining
Halothece sp. PCC7418 K9Y873
-
-
Oscillatoria nigro-viridis K9VK16
-
-
Oscillatoria nigro-viridis PCC7112 K9VK16
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrinogen IX + 3 O2
-
 Oscillatoria nigro-viridis protoporphyrin IX + 3 H2O2
-
 ?
protoporphyrinogen IX + 3 O2
-
 Halothece sp. PCC7418 protoporphyrin IX + 3 H2O2
-
 ?
protoporphyrinogen IX + 3 O2
-
 Oscillatoria nigro-viridis PCC7112 protoporphyrin IX + 3 H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 54000, SDS-PAGE Oscillatoria nigro-viridis
monomer 1 * 52000, SDS-PAGE Halothece sp. PCC7418
monomer 1 * 51100, calculated from amino acid sequence Halothece sp. PCC7418
monomer 1 * 53700, calculated from amino acid sequence Oscillatoria nigro-viridis

Synonyms

Synonyms Comment Organism
hemY
-
 Oscillatoria nigro-viridis
hemY
-
 Halothece sp. PCC7418
PPO
-
 Oscillatoria nigro-viridis
PPO
-
 Halothece sp. PCC7418
protoporphyrinogen IX oxidase
-
 Oscillatoria nigro-viridis
protoporphyrinogen IX oxidase
-
 Halothece sp. PCC7418

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.252
-
 protoporphyrinogen IX pH and temperature not specified in the publication Oscillatoria nigro-viridis
0.256
-
 protoporphyrinogen IX pH and temperature not specified in the publication Halothece sp. PCC7418

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Oscillatoria nigro-viridis
FAD
-
 Halothece sp. PCC7418

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000091
-
 pH and temperature not specified in the publication Halothece sp. PCC7418 tiafenacil
0.000012
-
 pH and temperature not specified in the publication Halothece sp. PCC7418 saflufenacil
0.000024
-
 pH and temperature not specified in the publication Oscillatoria nigro-viridis tiafenacil
0.0000484
-
 pH and temperature not specified in the publication Oscillatoria nigro-viridis saflufenacil

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
27.7
-
 protoporphyrinogen IX pH and temperature not specified in the publication Oscillatoria nigro-viridis
33.2
-
 protoporphyrinogen IX pH and temperature not specified in the publication Halothece sp. PCC7418