Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
protoporphyrinogen IX | acts as a competitive inhibitor of the coproporphyrinogen III to coproporphyrin oxidase activity of HemY | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bacillus subtilis | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + O2 | Bacillus subtilis | 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate | coproporphyrin III + H2O | - |
? | |
coproporphyrinogen III + O2 | Bacillus subtilis 3G18 | 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate | coproporphyrin III + H2O | - |
? | |
protoporphyrinogen IX + O2 | Bacillus subtilis | - |
protoporphyrin IX + H2O | - |
? | |
protoporphyrinogen IX + O2 | Bacillus subtilis 3G18 | - |
protoporphyrin IX + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis 3G18 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + O2 | 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate | Bacillus subtilis | coproporphyrin III + H2O | - |
? | |
coproporphyrinogen III + O2 | 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate | Bacillus subtilis 3G18 | coproporphyrin III + H2O | - |
? | |
additional information | the enzyme does not oxidize uroporphyrinogen III | Bacillus subtilis | ? | - |
? | |
additional information | the enzyme does not oxidize uroporphyrinogen III | Bacillus subtilis 3G18 | ? | - |
? | |
protoporphyrinogen IX + O2 | - |
Bacillus subtilis | protoporphyrin IX + H2O | - |
? | |
protoporphyrinogen IX + O2 | - |
Bacillus subtilis 3G18 | protoporphyrin IX + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Bacillus subtilis |
? | x * 51203, calculated from amino acid sequence | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
hemY | - |
Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
protoporphyrinogen IX | at pH 7.2 and 30°C | Bacillus subtilis |