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Literature summary for 1.3.3.15 extracted from

  • Hansson, M.; Hederstedt, L.
    Bacillus subtilis HemY is a peripheral membrane protein essential for protoheme IX synthesis which can oxidize coproporphyrinogen III and protoporphyrinogen IX (1994), J. Bacteriol., 176, 5962-5970.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
protoporphyrinogen IX acts as a competitive inhibitor of the coproporphyrinogen III to coproporphyrin oxidase activity of HemY Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Bacillus subtilis 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
x * 53000, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coproporphyrinogen III + O2 Bacillus subtilis 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate coproporphyrin III + H2O
-
?
coproporphyrinogen III + O2 Bacillus subtilis 3G18 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate coproporphyrin III + H2O
-
?
protoporphyrinogen IX + O2 Bacillus subtilis
-
protoporphyrin IX + H2O
-
?
protoporphyrinogen IX + O2 Bacillus subtilis 3G18
-
protoporphyrin IX + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 3G18
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen III + O2 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate Bacillus subtilis coproporphyrin III + H2O
-
?
coproporphyrinogen III + O2 12fold higher catalytic efficiency with coproporphyrinogen III compared to protoporphyrinogen as substrate Bacillus subtilis 3G18 coproporphyrin III + H2O
-
?
additional information the enzyme does not oxidize uroporphyrinogen III Bacillus subtilis ?
-
?
additional information the enzyme does not oxidize uroporphyrinogen III Bacillus subtilis 3G18 ?
-
?
protoporphyrinogen IX + O2
-
Bacillus subtilis protoporphyrin IX + H2O
-
?
protoporphyrinogen IX + O2
-
Bacillus subtilis 3G18 protoporphyrin IX + H2O
-
?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Bacillus subtilis
? x * 51203, calculated from amino acid sequence Bacillus subtilis

Synonyms

Synonyms Comment Organism
hemY
-
Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0003
-
protoporphyrinogen IX at pH 7.2 and 30Ā°C Bacillus subtilis