Literature summary for 1.3.3.15 extracted from

Qin, X.; Sun, L.; Wen, X.; Yang, X.; Tan, Y.; Jin, H.; Cao, Q.; Zhou, W.; Xi, Z.; Shen, Y.
Structural insight into unique properties of protoporphyrinogen oxidase from Bacillus subtilis (2010), J. Struct. Biol., 170, 76-82.

Search for more literature for 1.3.3.15

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with acifluorfen, sitting drop vapor diffusion method, using 2.4 M ammonium phosphate dibasic, 0.1 M Tris-HCl pH 7.5	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
F227R	the mutant exhibits slight decrease in catalysis compared to the wild type enzyme	Bacillus subtilis
I176A	the mutation leads to about 30fold decrease in catalytic efficiency compared to the wild type enzyme	Bacillus subtilis
K71A	the mutation leads to about 50fold decrease in catalytic efficiency compared to the wild type enzyme	Bacillus subtilis
P64A	the mutant exhibits a 14fold decrease in catalysis compared to the wild type enzyme	Bacillus subtilis
Y366N	the mutation leads to about 100fold decrease in catalytic efficiency compared to the wild type enzyme	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
acifluorfen	-	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Bacillus subtilis	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
coproporphyrinogen III + O2	Bacillus subtilis	-	coproporphyrin III + H2O	-	?
coproporphyrinogen III + O2	Bacillus subtilis 168	-	coproporphyrin III + H2O	-	?
protoporphyrinogen IX + O2	Bacillus subtilis	-	protoporphyrin IX + H2O	-	?
protoporphyrinogen IX + O2	Bacillus subtilis 168	-	protoporphyrin IX + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P32397	-	-
Bacillus subtilis 168	P32397	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and S200 gel filtration	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
coproporphyrinogen III + O2	-	Bacillus subtilis	coproporphyrin III + H2O	-	?
coproporphyrinogen III + O2	-	Bacillus subtilis 168	coproporphyrin III + H2O	-	?
protoporphyrinogen IX + O2	-	Bacillus subtilis	protoporphyrin IX + H2O	-	?
protoporphyrinogen IX + O2	-	Bacillus subtilis 168	protoporphyrin IX + H2O	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
PPO	-	Bacillus subtilis
protoporphyrinogen IX oxidase	-	Bacillus subtilis

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Bacillus subtilis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.03774	-	acifluorfen	wild type enzyme at pH 7.5, temperature not specified in the publication	Bacillus subtilis

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Release 2023.1 (January 2023)