Any feedback?
Literature summary for 1.3.3.15 extracted from
- Purification of and kinetic studies on a cloned protoporphyrinogen oxidase from the aerobic bacterium Bacillus subtilis (1998), Arch. Biochem. Biophys., 358, 251-256.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bilirubin | competitive inhibitor | Bacillus subtilis |  |
| Biliverdin | competitive inhibitor | Bacillus subtilis | |
| hemin | competitive inhibitor | Bacillus subtilis | |
| methylacifluorfen | weajk inhibition at 0.1 mM | Bacillus subtilis | |
| additional information | the enzyme is not inhibited by the diphenyl ether herbicide acifluorfen at 0.1 mM | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
protoporphyrinogen IX | at pH 8.7 and 37°C | Bacillus subtilis | |
| 0.00429 | - |
mesoporphyrinogen IX | at pH 8.7 and 37°C | Bacillus subtilis | |
| 0.00529 | - |
Coproporphyrinogen III | at pH 8.7 and 37°C | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coproporphyrinogen III + O2 | Bacillus subtilis | - |
coproporphyrin III + H2O | - |
? | |
| mesoporphyrinogen IX + O2 | Bacillus subtilis | - |
mesoporphyrin IX + H2O | - |
? | |
| protoporphyrinogen IX + O2 | Bacillus subtilis | - |
protoporphyrin IX + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ affinity column chromatography | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coproporphyrinogen III + O2 | - |
Bacillus subtilis | coproporphyrin III + H2O | - |
? | |
| mesoporphyrinogen IX + O2 | - |
Bacillus subtilis | mesoporphyrin IX + H2O | - |
? | |
| protoporphyrinogen IX + O2 | - |
Bacillus subtilis | protoporphyrin IX + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hemY | - |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
- |
Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Bacillus subtilis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus subtilis | isoelectric focusing | - |
7.5 |
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