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Literature summary for 1.3.3.15 extracted from

  • Corrigall, A.V.; Siziba, K.B.; Maneli, M.H.; Shephard, E.G.; Ziman, M.; Dailey, T.A.; Dailey, H.A.; Kirsch, R.E.; Meissner, P.N.
    Purification of and kinetic studies on a cloned protoporphyrinogen oxidase from the aerobic bacterium Bacillus subtilis (1998), Arch. Biochem. Biophys., 358, 251-256.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
bilirubin competitive inhibitor Bacillus subtilis
Biliverdin competitive inhibitor Bacillus subtilis
hemin competitive inhibitor Bacillus subtilis
methylacifluorfen weajk inhibition at 0.1 mM Bacillus subtilis
additional information the enzyme is not inhibited by the diphenyl ether herbicide acifluorfen at 0.1 mM Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
protoporphyrinogen IX at pH 8.7 and 37┬░C Bacillus subtilis
0.00429
-
mesoporphyrinogen IX at pH 8.7 and 37┬░C Bacillus subtilis
0.00529
-
Coproporphyrinogen III at pH 8.7 and 37┬░C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
x * 56000, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coproporphyrinogen III + O2 Bacillus subtilis
-
coproporphyrin III + H2O
-
?
mesoporphyrinogen IX + O2 Bacillus subtilis
-
mesoporphyrin IX + H2O
-
?
protoporphyrinogen IX + O2 Bacillus subtilis
-
protoporphyrin IX + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen III + O2
-
Bacillus subtilis coproporphyrin III + H2O
-
?
mesoporphyrinogen IX + O2
-
Bacillus subtilis mesoporphyrin IX + H2O
-
?
protoporphyrinogen IX + O2
-
Bacillus subtilis protoporphyrin IX + H2O
-
?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
hemY
-
Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FAD
-
Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus subtilis isoelectric focusing
-
7.5