Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL-21+ cells | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
H447G | the mutant is inactive and shows undetectable FMN binding | Trypanosoma cruzi |
K450G | the substitution results in an enzyme that retains an ability to bind FMN (around 50%of the wild type enzyme), but which exhibits no activity | Trypanosoma cruzi |
K55G | the mutation has only a minor inhibitory effect on both FMN binding and biochemical activity | Trypanosoma cruzi |
K55H | the mutation causes dramatically reduced FMN binding and enzyme activity (above 90%) | Trypanosoma cruzi |
K55L | the mutant is inactive and shows undetectable FMN binding | Trypanosoma cruzi |
W448G | the mutant is inactive and shows undetectable FMN binding | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | complete inhibition at 1 mM | Trypanosoma cruzi | |
N-ethylmaleimide | 77% inhibition at 1 mM | Trypanosoma cruzi | |
p-chloromercuribenzoate | complete inhibition at 1 mM | Trypanosoma cruzi | |
Zn2+ | 63% inhibition at 1 mM | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.82 | - |
D-arabinono-1,4-lactone | in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA | Trypanosoma cruzi | |
11.22 | - |
L-galactono-1,4-lactone | in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
SDS-PAGE | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Trypanosoma cruzi | - |
epimastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinono-1,4-lactone + O2 | - |
Trypanosoma cruzi | ? | - |
? | |
L-galactono-1,4-lactone + O2 | - |
Trypanosoma cruzi | L-ascorbate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAL | - |
Trypanosoma cruzi |
galactonolactone oxidase | - |
Trypanosoma cruzi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
heat-inactivated above 50°C | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.161 | - |
L-galactono-1,4-lactone | in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA | Trypanosoma cruzi | |
0.285 | - |
D-arabinono-1,4-lactone | in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | dependent on non-covalently-bound FMN | Trypanosoma cruzi |