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Literature summary for 1.3.3.11 extracted from

  • Toyama, H.; Nishibayashi, E.; Saeki, M.; Adachi, O.; Matsushita, K.
    Factors required for the catalytic reaction of PqqC/D which produces pyrroloquinoline quinone (2007), Biochem. Biophys. Res. Commun., 354, 290-295.
    View publication on PubMed
Search for more literature for 1.3.3.11

Activating Compound

Activating Compound Comment Organism Structure
activating factor ActF consists of a cytosolic fraction, activating factor ActF enhances pyrroloquinoline quinone synthesis in the presence of NAD(P)H Escherichia coli
activating factor ActF consists of a cytosolic fraction, activating factor ActF enhances pyrroloquinoline quinone synthesis in the presence of NAD(P)H Methylorubrum extorquens
dithioerythritol
-
 Klebsiella pneumoniae
dithioerythritol
-
 Methylorubrum extorquens
dithiothreitol
-
 Klebsiella pneumoniae
dithiothreitol
-
 Methylorubrum extorquens
dithiothreitol 0.1 mM enhances pyrroloquinoline quinone production Escherichia coli
dithiothreitol 0.1 mM enhances pyrroloquinoline quinone production Methylorubrum extorquens
NAD(P)H NAD(P)H enhances pyrroloquinoline quinone production in the presence of activating factor ActF Escherichia coli
NAD(P)H NAD(P)H enhances pyrroloquinoline quinone production in the presence of activating factor ActF Methylorubrum extorquens
TrxAB the complete TrxAB system enhance the pyrroloquinoline quinone production Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
oxidized pyrroloquinoline quinone in vitro pyrroloquinoline quinone is oxidized by molecular oxygen and then the oxidized pyrroloquinoline quinone is trapped in PqqC/D to show product inhibition Escherichia coli
oxidized pyrroloquinoline quinone in vitro pyrroloquinoline quinone is oxidized by molecular oxygen and then the oxidized pyrroloquinoline quinone is trapped in PqqC/D to show product inhibition Methylorubrum extorquens
pyrroloquinoline quinone
-
 Escherichia coli
pyrroloquinoline quinone
-
 Klebsiella pneumoniae
pyrroloquinoline quinone
-
 Methylorubrum extorquens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 2 * 29000, SDS-PAGE Escherichia coli
29000
-
 2 * 29000, SDS-PAGE Methylorubrum extorquens
58000
-
 SDS-PAGE Escherichia coli
58000
-
 SDS-PAGE Methylorubrum extorquens
150000
-
 determined by gel filtration, with DTT Methylorubrum extorquens
300000
-
 determined by gel filtration, without DTT Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2 Escherichia coli
-
 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2 Klebsiella pneumoniae
-
 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2 Methylorubrum extorquens
-
 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
additional information Methylorubrum extorquens PqqC/D produces the reduced pyrroloquinoline quinone from the intermediate in vivo ?
-
 ?
additional information Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 PqqC/D produces the reduced pyrroloquinoline quinone from the intermediate in vivo ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Klebsiella pneumoniae
-
-
-
Methylorubrum extorquens
-
-
-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Methylorubrum extorquens
Superdex S-200 gel filtration Escherichia coli
Superdex S-200 gel filtration Methylorubrum extorquens

Reaction

Reaction Comment Organism Reaction ID
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate + 3 O2 = 4,5-dioxo-4,5-dihydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + 2 H2O2 + 2 H2O PqqC/D produces the reduced pyrroloquinoline quinone from the intermediate in vivo Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2
-
 Escherichia coli 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2
-
 Methylorubrum extorquens 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2
-
 Escherichia coli 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2
-
 Klebsiella pneumoniae 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2
-
 Methylorubrum extorquens 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,5,6,7,8,-octahydroquinoline-2,4-dicarboxylate + O2
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 4,5-dioxo-3a,4,5,6,7,8,9,9b-octahydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O
-
 ?
additional information PqqC/D produces the reduced pyrroloquinoline quinone from the intermediate in vivo Methylorubrum extorquens ?
-
 ?
additional information PqqC/D has low affinity to PQQH2 Escherichia coli ?
-
 ?
additional information PqqC/D has low affinity to PQQH2 Methylorubrum extorquens ?
-
 ?
additional information PqqC/D produces the reduced pyrroloquinoline quinone from the intermediate in vivo Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 ?
-
 ?
additional information PqqC/D has low affinity to PQQH2 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 29000, SDS-PAGE Escherichia coli
homodimer 2 * 29000, SDS-PAGE Methylorubrum extorquens

Synonyms

Synonyms Comment Organism
PqqC
-
 Escherichia coli
PqqC
-
 Klebsiella pneumoniae
PqqC
-
 Methylorubrum extorquens
PqqC/D
-
 Escherichia coli
PqqC/D
-
 Klebsiella pneumoniae
PqqC/D
-
 Methylorubrum extorquens
pyrroloquinoline-quinone synthase
-
 Escherichia coli
pyrroloquinoline-quinone synthase
-
 Klebsiella pneumoniae
pyrroloquinoline-quinone synthase
-
 Methylorubrum extorquens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 activity assay Escherichia coli
25
-
 activity assay Klebsiella pneumoniae
25
-
 activity assay Methylorubrum extorquens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
 activity assay Escherichia coli
8.3
-
 activity assay Klebsiella pneumoniae
8.3
-
 activity assay Methylorubrum extorquens