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Literature summary for 1.3.2.3 extracted from

  • Schimmeyer, J.; Bock, R.; Meyer, E.H.
    L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis (2016), Plant Mol. Biol., 90, 117-126 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.2.3

Protein Variants

Protein Variants Comment Organism
additional information analysis of the vtc2-1 mutant shows that complex I assembly is not affected in this mutant, while it is affected in the mutant ndufs4 of NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 of Arabidopsis thaliana Col-0, the enzyme is located in the 400 and 450 kDa carbonic anhydrase-containing complexes accumulating in the ndufs4 mutant. Very low amounts of complex I and other assembly intermediates in the gldh mutant, suggesting that the assembly of complex I is not completely arrested, but it is severely impaired in the absence of GLDH Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane GLDH behaves as an integral protein of the inner membrane facing the intermembrane space Arabidopsis thaliana 5743
-
additional information in a mutant ndufs4 of NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 of Arabidopsis thaliana Col-0, the enzyme is located in the 400 and 450 kDa carbonic anhydrase-containing complexes accumulating in the ndufs4 mutant Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactono-1,4-lactone + 4 ferricytochrome c Arabidopsis thaliana overall reaction L-dehydroascorbate + 4 ferrocytochrome c + 4 H+
-
 ?
L-galactono-1,4-lactone + 4 ferricytochrome c Arabidopsis thaliana Col-0 overall reaction L-dehydroascorbate + 4 ferrocytochrome c + 4 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SU56
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-
Arabidopsis thaliana Col-0 Q9SU56
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactono-1,4-lactone + 4 ferricytochrome c overall reaction Arabidopsis thaliana L-dehydroascorbate + 4 ferrocytochrome c + 4 H+
-
 ?
L-galactono-1,4-lactone + 4 ferricytochrome c overall reaction Arabidopsis thaliana Col-0 L-dehydroascorbate + 4 ferrocytochrome c + 4 H+
-
 ?

Synonyms

Synonyms Comment Organism
GLDH
-
 Arabidopsis thaliana
L-galactono-1,4-lactone dehydrogenase
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
 Arabidopsis thaliana
FAD
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction analysis of the vtc2-1 mutant shows that complex I assembly is not affected in this mutant, while it is affected in the mutant ndufs4 of NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 of Arabidopsis thaliana Col-0, in ndufs4, complex I is not assembled and GLDH is not present in any intermediate larger than 450 kDa. This suggests that the 450 kDa intermediate is a precursor of complex I. Very low amounts of complex I and other assembly intermediates in the gldh mutant, suggesting that the assembly of complex I is not completely arrested, but it is severely impaired in the absence of GLDH Arabidopsis thaliana
metabolism the enzyme catalyzes the last step of ascorbate synthesis by oxidising L-galactone-1,4-lactone to ascorbate and transferring two electrons to cytochrome c Arabidopsis thaliana
physiological function the enzyme can play two distinct roles during complex I assembly. First, it can play a structural or stabilizing role for specific assembly intermediates. Second, it can indirectly be essential through providing the ascorbate that might be required during the assembly process. Enzyme GLDH is not required for the early stages of complex I assembly, but it is important for at least one step of the assembly process (transition from the 200 kDa intermediate to the 400 kDa intermediate), it is associated with some assembly intermediates, but it is absent from the mature complex Arabidopsis thaliana