BRENDA - Enzyme Database
show all sequences of 1.3.1.B13

Polyprenol reductase2 deficiency is lethal in Arabidopsis due to male sterility

Jozwiak, A.; Gutkowska, M.; Gawarecka, K.; Surmacz, L.; Buczkowska, A.; Lichocka, M.; Nowakowska, J.; Swiezewska, E.; Plant Cell 27, 3336-3353 (2015)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
recombinant expression of isozyme PPRD1 in Saccharomyces cerevisiae strains BY4741, L5366, and mutant strains dfg10DELTA and dfg10-100 from lasmid pYES-DEST52, recombinant expression of isozyme PPRD1 in Escherichia coli strain BL21, method optimization. Agrobacterium-mediated transient coexpression of GFP-tagged isozyme with plant organelle markers (mCherry fusions) in Nicotiana benthamiana. Quantitative PCR enzyme expression and promoter activity analysis
Arabidopsis thaliana
recombinant expression of isozyme PPRD1 in Saccharomyces cerevisiae strains BY4741, L5366, and mutant strains dfg10DELTA and dfg10-100 from lasmid pYES-DEST52, recombinant expression of wild-type an dmutant isozyme PPRD1s in Escherichia coli strain BL21, method optimization. Agrobacterium-mediated transient coexpression of GFP-tagged isozyme with plant organelle markers (mCherry fusions) in Nicotiana benthamiana. Quantitative PCR enzyme expression and promoter activity analysis
Arabidopsis thaliana
Engineering
Protein Variants
Commentary
Organism
H321L
site-directed mutagenesis, the mutant shows 47% of wild-type reductase activity
Arabidopsis thaliana
H336L
site-directed mutagenesis, the mutant shows 45% of wild-type reductase activity
Arabidopsis thaliana
additional information
PPRD2 mutants with His321 or His336, corresponding to His296 or His309 in human hSRD5A3, substituted by Leu are employed to complement the yeast dfg10DELTA mutant. Construction of two heterozygous T-DNA PPRD2 insertion lines, pprd2-1 (SALK_113221C) and pprd2-2 (SALK_006421), PPRD2 deficiency is lethal in Arabidopsis thaliana due to male sterility, PPRD2 knockout plants are not viable. For the pprd2 homozygous mutants, consistently with their Dol deficiency, defects in N-glycosylation are accompanied by degradation of some proteins, e.g., SKU5
Arabidopsis thaliana
additional information
total loss of activity of the putative C-terminally truncated PPRD1 proteins encoded by PPRD1-INT3 and PPRD1-INT4
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Arabidopsis thaliana
5783
-
additional information
polyprenol reductases are diversely localized within the plant cell, isozyme expression analysis, overview
Arabidopsis thaliana
-
-
plasma membrane
-
Arabidopsis thaliana
5886
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a polyprenol + NADPH + H+
Arabidopsis thaliana
-
a dolichol + NADP+
-
-
?
a polyprenol + NADPH + H+
Arabidopsis thaliana Col-0
-
a dolichol + NADP+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q9CAH5
-
-
Arabidopsis thaliana
Q9SI62
-
-
Arabidopsis thaliana Col-0
Q9CAH5
-
-
Arabidopsis thaliana Col-0
Q9SI62
-
-
no activity in Escherichia coli
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Arabidopsis thaliana
-
flower bud
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PPRD1 is expressed in young seedlings, while in older plants, it is mainly expressed in the roots and flowers. Expression of PPRD1 increases in the roots and decreases in the leaves. Isozyme expression analysis, overview
Arabidopsis thaliana
-
additional information
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PRD2 is expressed in all organs analyzed in young seedlings and older plants. With age, expression of PPRD2 is fairly constant in the roots and decreases in the leaves. Isozyme expression analysis, overview
Arabidopsis thaliana
-
pollen
only isozyme PPRD2
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
a polyprenol + NADPH + H+
-
746028
Arabidopsis thaliana
a dolichol + NADP+
-
-
-
?
a polyprenol + NADPH + H+
-
746028
Arabidopsis thaliana Col-0
a dolichol + NADP+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
polyprenol reductase 1
-
Arabidopsis thaliana
polyprenol reductase 2
-
Arabidopsis thaliana
polyprenol reductase2
-
Arabidopsis thaliana
PPRD1
-
Arabidopsis thaliana
PPRD2
-
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of isozyme PPRD1 in Saccharomyces cerevisiae strains BY4741, L5366, and mutant strains dfg10DELTA and dfg10-100 from lasmid pYES-DEST52, recombinant expression of isozyme PPRD1 in Escherichia coli strain BL21, method optimization. Agrobacterium-mediated transient coexpression of GFP-tagged isozyme with plant organelle markers (mCherry fusions) in Nicotiana benthamiana. Quantitative PCR enzyme expression and promoter activity analysis
Arabidopsis thaliana
recombinant expression of isozyme PPRD1 in Saccharomyces cerevisiae strains BY4741, L5366, and mutant strains dfg10DELTA and dfg10-100 from lasmid pYES-DEST52, recombinant expression of wild-type an dmutant isozyme PPRD1s in Escherichia coli strain BL21, method optimization. Agrobacterium-mediated transient coexpression of GFP-tagged isozyme with plant organelle markers (mCherry fusions) in Nicotiana benthamiana. Quantitative PCR enzyme expression and promoter activity analysis
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Engineering (protein specific)
Protein Variants
Commentary
Organism
H321L
site-directed mutagenesis, the mutant shows 47% of wild-type reductase activity
Arabidopsis thaliana
H336L
site-directed mutagenesis, the mutant shows 45% of wild-type reductase activity
Arabidopsis thaliana
additional information
PPRD2 mutants with His321 or His336, corresponding to His296 or His309 in human hSRD5A3, substituted by Leu are employed to complement the yeast dfg10DELTA mutant. Construction of two heterozygous T-DNA PPRD2 insertion lines, pprd2-1 (SALK_113221C) and pprd2-2 (SALK_006421), PPRD2 deficiency is lethal in Arabidopsis thaliana due to male sterility, PPRD2 knockout plants are not viable. For the pprd2 homozygous mutants, consistently with their Dol deficiency, defects in N-glycosylation are accompanied by degradation of some proteins, e.g., SKU5
Arabidopsis thaliana
additional information
total loss of activity of the putative C-terminally truncated PPRD1 proteins encoded by PPRD1-INT3 and PPRD1-INT4
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Arabidopsis thaliana
5783
-
additional information
polyprenol reductases are diversely localized within the plant cell, isozyme expression analysis, overview
Arabidopsis thaliana
-
-
plasma membrane
-
Arabidopsis thaliana
5886
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a polyprenol + NADPH + H+
Arabidopsis thaliana
-
a dolichol + NADP+
-
-
?
a polyprenol + NADPH + H+
Arabidopsis thaliana Col-0
-
a dolichol + NADP+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Arabidopsis thaliana
-
flower bud
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PPRD1 is expressed in young seedlings, while in older plants, it is mainly expressed in the roots and flowers. Expression of PPRD1 increases in the roots and decreases in the leaves. Isozyme expression analysis, overview
Arabidopsis thaliana
-
additional information
polyprenol reductases are expressed in a tissue-specific manner in Arabidopsis thaliana. Isozyme PRD2 is expressed in all organs analyzed in young seedlings and older plants. With age, expression of PPRD2 is fairly constant in the roots and decreases in the leaves. Isozyme expression analysis, overview
Arabidopsis thaliana
-
pollen
only isozyme PPRD2
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
a polyprenol + NADPH + H+
-
746028
Arabidopsis thaliana
a dolichol + NADP+
-
-
-
?
a polyprenol + NADPH + H+
-
746028
Arabidopsis thaliana Col-0
a dolichol + NADP+
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
the two genes PPRD1 and PPRD2 are orthologous to human SRD5A3 (steroid 5alpha reductase type 3) and encode polyprenol reductases responsible for conversion of polyprenol to dolichol in Arabidopsis thaliana
Arabidopsis thaliana
malfunction
polyprenol reductase2 deficiency is lethal in Arabidopsis thaliana due to male sterility, PPRD2 knockout plants are not viable. PPRD2 deficiency results in aberrant development of the male gametophyte and sporophyte. Impaired protein glycosylation seems to be the major factor underlying these defects although disturbances in other cellular dolichol-dependent processes might also contribute. Shortage of dolichol in PPRD2-deficient cells is partially rescued by PPRD1 overexpression or by supplementation with dolichol. Supplementation of the human diet with dolichol-enriched plant tissues can allow therapeutic interventions in glycosylation disorders
Arabidopsis thaliana
additional information
the catalytic 3-oxo-5alpha-steroid 4-dehydrogenase domain of PPRD is localized at the C-terminus
Arabidopsis thaliana
additional information
the catalytic 3-oxo-5alpha-steroid domain of PPRD is localized at the C-terminus. Besides His321 or His336, other residues of PPRD2 might be involved in the reduction
Arabidopsis thaliana
physiological function
isozyme PPRD2 is essential for plant viability
Arabidopsis thaliana
physiological function
isozymes PPRD1 and PPRD2 are the factors mediating the key step of the dolichol cycle in plant cells. Shortage of dolichol in PPRD2-deficient cells is partially rescued by PPRD1 overexpression
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
evolution
the two genes PPRD1 and PPRD2 are orthologous to human SRD5A3 (steroid 5alpha reductase type 3) and encode polyprenol reductases responsible for conversion of polyprenol to dolichol in Arabidopsis thaliana
Arabidopsis thaliana
malfunction
polyprenol reductase2 deficiency is lethal in Arabidopsis thaliana due to male sterility, PPRD2 knockout plants are not viable. PPRD2 deficiency results in aberrant development of the male gametophyte and sporophyte. Impaired protein glycosylation seems to be the major factor underlying these defects although disturbances in other cellular dolichol-dependent processes might also contribute. Shortage of dolichol in PPRD2-deficient cells is partially rescued by PPRD1 overexpression or by supplementation with dolichol. Supplementation of the human diet with dolichol-enriched plant tissues can allow therapeutic interventions in glycosylation disorders
Arabidopsis thaliana
additional information
the catalytic 3-oxo-5alpha-steroid 4-dehydrogenase domain of PPRD is localized at the C-terminus
Arabidopsis thaliana
additional information
the catalytic 3-oxo-5alpha-steroid domain of PPRD is localized at the C-terminus. Besides His321 or His336, other residues of PPRD2 might be involved in the reduction
Arabidopsis thaliana
physiological function
isozyme PPRD2 is essential for plant viability
Arabidopsis thaliana
physiological function
isozymes PPRD1 and PPRD2 are the factors mediating the key step of the dolichol cycle in plant cells. Shortage of dolichol in PPRD2-deficient cells is partially rescued by PPRD1 overexpression
Arabidopsis thaliana
Other publictions for EC 1.3.1.B13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743987
Wheeler
SRD5A3-CDG Expanding the phe ...
Homo sapiens
Am. J. Med. Genet. A
170
3165-3171
2016
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1
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1
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1
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2
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1
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1
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1
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1
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-
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-
-
-
-
-
-
2
2
-
-
-
746028
Jozwiak
Polyprenol reductase2 deficie ...
no activity in Escherichia coli, Arabidopsis thaliana, Arabidopsis thaliana Col-0
Plant Cell
27
3336-3353
2015
-
-
2
-
4
-
-
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4
-
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4
-
13
-
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10
-
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4
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6
-
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2
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2
2
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4
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4
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4
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10
-
-
4
-
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-
-
-
-
-
-
-
-
6
6
-
-
-
719256
Cantagrel
SRD5A3 is required for convert ...
Homo sapiens
Cell
142
203-217
2010
-
-
1
-
1
-
-
-
-
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1
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1
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2
-
1
1
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1
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1
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1
1
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1
-
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-
-
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1
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-
-
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-
-
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2
-
1
-
-
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1
-
-
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2
2
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