Cloned (Comment) | Organism |
---|---|
overexpression of protein in Escherichia coli strain JM109 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-NADP+ | noncompetitive with respect to NADPH | Escherichia coli | |
3-acetylpyridine adenine dinucleotide phosphate | oxidized form, competitive with respect to NADPH | Escherichia coli | |
ADP | at high concentration, competitive with respect to NADPH | Escherichia coli | |
ADP-ribose | at high concentration, competitive with respect to NADPH | Escherichia coli | |
NADP+ | noncompetitive with respect to NADPH | Escherichia coli | |
NADPH | substrate inhibition at pH 7.0, Ki: 1.6 mM | Escherichia coli | |
nicotinamide 1,N6-ethenoadenine dinucleotide phosphate | oxidized form, noncompetitive with respect to NADPH | Escherichia coli | |
nicotinamide hypoxanthine dinucleotide phosphate | oxidized form, noncompetitive with respect to NADPH | Escherichia coli | |
thio-NADP+ | noncompetitive with respect to NADPH | Escherichia coli | |
UDP | at high concentration, noncompetitive with respect to NADPH | Escherichia coli | |
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine | substrate inhibition at pH 6, Ki 0.01 mM with 0.15 mM NADPH, weaker at more basic pH | Escherichia coli | |
uridine diphospho-N-acetylglucosamine | at very high concentration, noncompetitive with respect to NADPH | Escherichia coli | |
uridine diphospho-N-acetylmuramic acid | uncompetitive with respect to NADPH | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine | in the presence of 50 mM K+ | Escherichia coli | |
0.022 | - |
NADPH | in the presence of 50 mM K+ | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | at saturating cation concentration maximal enzyme activity: 2 units/mg | Escherichia coli | |
K+ | at saturating cation concentration maximal enzyme activity: 50 units/mg | Escherichia coli | |
K+ | without monovalent cations, only minimium enzyme activity | Escherichia coli | |
additional information | divalent cations have no activating effect | Escherichia coli | |
Na+ | at saturating cation concentration maximal enzyme activity: 5 units/mg | Escherichia coli | |
NH4+ | at saturating cation concentration maximal enzyme activity: 35 units/mg | Escherichia coli | |
Rb+ | at saturating cation concentration maximal enzyme activity: 33 units/mg | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38340 | - |
electrospray mass spectrometry of recombinant protein | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | Escherichia coli | - |
UDP-N-acetyl-3-D-muramate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | - |
Escherichia coli | UDP-N-acetyl-3-D-muramate + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol FAD per mol enzyme bound | Escherichia coli | |
additional information | NADH not substrate | Escherichia coli | |
additional information | thio-NADPH not substrate | Escherichia coli | |
NADPH | - |
Escherichia coli |