Application | Comment | Organism |
---|---|---|
synthesis | an expression system for production of high levels of botryococcene in Nicotiana tabacum plants can be used involving the recombinant chimeric enzyme, trichome-specific expression of botryococcene metabolism, overview | Botryococcus braunii |
Cloned (Comment) | Organism |
---|---|
gene SSL-3, recombinant expression of the enzyme in Nicotiana tabacum, ene constructs consisted of a peptide fusion of SSL-1 and SSL-3 connected by a triplet repeat peptide linker of Gly-Gly-Ser-Gly, with or without appending the C-terminal end (71 amino acids) of the Botryococcus braunii squalene synthase onto the C-terminus of SSL-3 and the FPS gene. The chimeric SSL1-3 genes and FPS gene are inserted downstream of the strong constitutive promoters Pcv and Pca, respectively. For trichome-specific expression of triterpene biosynthesis, the trichome-specific promoters Pcbt and Pcyp16 are fused to 5' end of botryococcene synthase gene, presqualene diphosphate synthase gene, and the FPS gene, respectively | Botryococcus braunii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of an expression system for production of high levels of botryococcene in Nicotiana tabacum strain KY 1068 plants by diverting carbon flux from the cytosolic mevalonate pathway or the plastidic methylerythritol phosphate pathway by the targeted overexpression of an avian farnesyl diphosphate synthase along with botryococcene synthase SSL-3 and presqualene diphosphate synthase SSL-1, overview. The structure of purified botryococcene from tobacco is determined by 1H-NMR and 13C-NMR spectral analyses. Modifications of botryococcene in tobacco by coexpressed triterpene methyltransferases (TMTs) from Botryococcus braunii | Botryococcus braunii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
presqualene diphosphate + NADPH + H+ | Botryococcus braunii | - |
C30 botryococcene + NADP+ + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Botryococcus braunii | G0Y288 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
presqualene diphosphate + NADPH + H+ | - |
Botryococcus braunii | C30 botryococcene + NADP+ + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SSL-3 | - |
Botryococcus braunii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Botryococcus braunii |