BRENDA - Enzyme Database
show all sequences of 1.3.1.96

Characterization of botryococcene synthase enzyme activity, a squalene synthase-like activity from the green microalga Botryococcus braunii, Race B

Okada, S.; Devarenne, T.P.; Murakami, M.; Abe, H.; Chappell, J.; Arch. Biochem. Biophys. 422, 110-118 (2004)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Tween 80
increasing concentrations of Tween 80 up to 2% enhance enzyme activity approximately 2.5fold
Botryococcus braunii
Inhibitors
Inhibitors
Commentary
Organism
Structure
squalestatin
-
Botryococcus braunii
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Botryococcus braunii
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
required for activity
Botryococcus braunii
Mg2+
required for activity
Botryococcus braunii
Mn2+
required for activity
Botryococcus braunii
Organism
Organism
UniProt
Commentary
Textmining
Botryococcus braunii
-
race B
-
Botryococcus braunii Berkeley (Showa)
-
race B
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
presqualene diphosphate + NADPH + H+
-
718688
Botryococcus braunii
squalene + diphosphate + NADP+
-
-
-
?
presqualene diphosphate + NADPH + H+
-
718688
Botryococcus braunii Berkeley (Showa)
squalene + diphosphate + NADP+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
squalene synthase
-
Botryococcus braunii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Botryococcus braunii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
in MOPS buffer
Botryococcus braunii
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Botryococcus braunii
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.000001
-
squalestatin
at pH 7.3 and 37°C
Botryococcus braunii
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Tween 80
increasing concentrations of Tween 80 up to 2% enhance enzyme activity approximately 2.5fold
Botryococcus braunii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Botryococcus braunii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
squalestatin
-
Botryococcus braunii
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.000001
-
squalestatin
at pH 7.3 and 37°C
Botryococcus braunii
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Botryococcus braunii
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
required for activity
Botryococcus braunii
Mg2+
required for activity
Botryococcus braunii
Mn2+
required for activity
Botryococcus braunii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
presqualene diphosphate + NADPH + H+
-
718688
Botryococcus braunii
squalene + diphosphate + NADP+
-
-
-
?
presqualene diphosphate + NADPH + H+
-
718688
Botryococcus braunii Berkeley (Showa)
squalene + diphosphate + NADP+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Botryococcus braunii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
in MOPS buffer
Botryococcus braunii
Other publictions for EC 1.3.1.96
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741903
Bell
Structure-function mapping of ...
Botryococcus braunii
Biochemistry
53
7570-7581
2014
-
-
1
-
2
-
-
-
1
1
-
2
-
1
-
-
-
-
-
-
-
-
3
-
1
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
2
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
720061
Niehaus
Functional identification of t ...
Botryococcus braunii
J. Biol. Chem.
287
8163-8173
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
720917
Niehaus
Identification of unique mecha ...
Botryococcus braunii
Proc. Natl. Acad. Sci. USA
108
12260-12265
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
718688
Okada
Characterization of botryococc ...
Botryococcus braunii, Botryococcus braunii Berkeley (Showa)
Arch. Biochem. Biophys.
422
110-118
2004
1
-
-
-
-
-
1
-
1
3
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
1
-
-
-
1
-
-
1
1
-
-
1
-
-
1
-
-
-
-
1
1
-
1
3
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
489850
Okada
Molecular characterization of ...
Botryococcus braunii, Botryococcus braunii Berkeley
Arch. Biochem. Biophys.
373
307-317
2000
-
-
1
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-