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show all sequences of 1.3.1.93

Dual functions of the trans-2-enoyl-CoA reductase TER in the sphingosine 1-phosphate metabolic pathway and in fatty acid elongation

Wakashima, T.; Abe, K.; Kihara, A.; J. Biol. Chem. 289, 24736-24748 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene TECR
Rattus norvegicus
gene TECR, cloning from liver cDNA, Ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells
Homo sapiens
gene TSC13, recombinant expression as N-terminal FLAG3-tagged enzyme from pAKNF313 and pAKNF315, or as N-terminal HA2-tagged enzyme
Saccharomyces cerevisiae
Engineering
Amino acid exchange
Commentary
Organism
additional information
HeLa cells are transfected with control siRNA or TER si RNA, siRNA-generated enzyme knockout mutant. Knockdown of TER in HeLa cells causes decreased sphingosine 1-phosphate metabolism in vitro and a reduction in the dihydrosphingosine metabolism
Homo sapiens
additional information
YRF50 cells (BY4741,pTSC13::KanMX4-tTA-ptetO7) are constructed by replacing the promoter of the TSC13 gene (pTSC13) with tetO7 promoter (ptetO7) using the KanMX4-tTA-ptetO7 cassette from the pCM225 plasmid. Strains ABY83 and ABY80 cells are constructed by deletion of the TSC13 gene in BY4741 cells bearing the pTW6 or pAB119 plasmid, respectively, using a tsc13DELTA::LEU2 fragment by homologous recombination. Generation of Saccharomyces cerevisiae Tsc13-lowered cells
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Homo sapiens
5783
-
endoplasmic reticulum
-
Rattus norvegicus
5783
-
endoplasmic reticulum
-
Saccharomyces cerevisiae
5783
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Saccharomyces cerevisiae
-
a very-long-chain acyl-CoA + NADP+
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Homo sapiens
-
a very-long-chain acyl-CoA + NADP+
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Rattus norvegicus
-
a very-long-chain acyl-CoA + NADP+
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Saccharomyces cerevisiae BY4741
-
a very-long-chain acyl-CoA + NADP+
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
Saccharomyces cerevisiae
-
palmitoyl-CoA + NADP+
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
Homo sapiens
-
palmitoyl-CoA + NADP+
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
Rattus norvegicus
-
palmitoyl-CoA + NADP+
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
Saccharomyces cerevisiae BY4741
-
palmitoyl-CoA + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q9NZ01
-
-
Rattus norvegicus
Q64232
-
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BY4741
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
HeLa cell
-
Homo sapiens
-
Hep-G2 cell
-
Homo sapiens
-
hepatocyte
-
Homo sapiens
-
IEC-6 cell
-
Rattus norvegicus
-
intestinal epithelial cell
-
Rattus norvegicus
-
neuronal cell
-
Rattus norvegicus
-
PC-12 cell
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae
a very-long-chain acyl-CoA + NADP+
-
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Homo sapiens
a very-long-chain acyl-CoA + NADP+
-
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Rattus norvegicus
a very-long-chain acyl-CoA + NADP+
-
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae BY4741
a very-long-chain acyl-CoA + NADP+
-
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae
palmitoyl-CoA + NADP+
-
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Homo sapiens
palmitoyl-CoA + NADP+
-
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Rattus norvegicus
palmitoyl-CoA + NADP+
-
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae BY4741
palmitoyl-CoA + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Homo sapiens
NADPH
-
Rattus norvegicus
NADPH
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
gene TECR
Rattus norvegicus
gene TECR, cloning from liver cDNA, Ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells
Homo sapiens
gene TSC13, recombinant expression as N-terminal FLAG3-tagged enzyme from pAKNF313 and pAKNF315, or as N-terminal HA2-tagged enzyme
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Homo sapiens
NADPH
-
Rattus norvegicus
NADPH
-
Saccharomyces cerevisiae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
HeLa cells are transfected with control siRNA or TER si RNA, siRNA-generated enzyme knockout mutant. Knockdown of TER in HeLa cells causes decreased sphingosine 1-phosphate metabolism in vitro and a reduction in the dihydrosphingosine metabolism
Homo sapiens
additional information
YRF50 cells (BY4741,pTSC13::KanMX4-tTA-ptetO7) are constructed by replacing the promoter of the TSC13 gene (pTSC13) with tetO7 promoter (ptetO7) using the KanMX4-tTA-ptetO7 cassette from the pCM225 plasmid. Strains ABY83 and ABY80 cells are constructed by deletion of the TSC13 gene in BY4741 cells bearing the pTW6 or pAB119 plasmid, respectively, using a tsc13DELTA::LEU2 fragment by homologous recombination. Generation of Saccharomyces cerevisiae Tsc13-lowered cells
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Homo sapiens
5783
-
endoplasmic reticulum
-
Rattus norvegicus
5783
-
endoplasmic reticulum
-
Saccharomyces cerevisiae
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Saccharomyces cerevisiae
-
a very-long-chain acyl-CoA + NADP+
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Homo sapiens
-
a very-long-chain acyl-CoA + NADP+
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Rattus norvegicus
-
a very-long-chain acyl-CoA + NADP+
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Saccharomyces cerevisiae BY4741
-
a very-long-chain acyl-CoA + NADP+
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
Saccharomyces cerevisiae
-
palmitoyl-CoA + NADP+
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
Homo sapiens
-
palmitoyl-CoA + NADP+
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
Rattus norvegicus
-
palmitoyl-CoA + NADP+
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
Saccharomyces cerevisiae BY4741
-
palmitoyl-CoA + NADP+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
HeLa cell
-
Homo sapiens
-
Hep-G2 cell
-
Homo sapiens
-
hepatocyte
-
Homo sapiens
-
IEC-6 cell
-
Rattus norvegicus
-
intestinal epithelial cell
-
Rattus norvegicus
-
neuronal cell
-
Rattus norvegicus
-
PC-12 cell
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae
a very-long-chain acyl-CoA + NADP+
-
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Homo sapiens
a very-long-chain acyl-CoA + NADP+
-
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Rattus norvegicus
a very-long-chain acyl-CoA + NADP+
-
-
-
?
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae BY4741
a very-long-chain acyl-CoA + NADP+
-
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae
palmitoyl-CoA + NADP+
-
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Homo sapiens
palmitoyl-CoA + NADP+
-
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Rattus norvegicus
palmitoyl-CoA + NADP+
-
-
-
?
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae BY4741
palmitoyl-CoA + NADP+
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
in membrane fractions prepared from TER siRNA-treated HeLa cells, the conversion of trans-2-hexadecenoyl-CoA to palmitoyl-CoA is largely impaired, and only a small amount of palmitoyl-CoA is produced. Instead, trans-2-hexadecenoyl-CoA is the main product, and C14:0-CoA is also detected
Homo sapiens
malfunction
ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells causes recovery in the deficient sphingosine 1-phosphate metabolic pathway, lethality of VLCFA-deficient mutations
Saccharomyces cerevisiae
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview. Ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells causes recovery in the deficient sphingosine 1-phosphate metabolic pathway
Homo sapiens
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview
Rattus norvegicus
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview
Saccharomyces cerevisiae
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Homo sapiens
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Rattus norvegicus
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Saccharomyces cerevisiae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
in membrane fractions prepared from TER siRNA-treated HeLa cells, the conversion of trans-2-hexadecenoyl-CoA to palmitoyl-CoA is largely impaired, and only a small amount of palmitoyl-CoA is produced. Instead, trans-2-hexadecenoyl-CoA is the main product, and C14:0-CoA is also detected
Homo sapiens
malfunction
ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells causes recovery in the deficient sphingosine 1-phosphate metabolic pathway, lethality of VLCFA-deficient mutations
Saccharomyces cerevisiae
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview. Ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells causes recovery in the deficient sphingosine 1-phosphate metabolic pathway
Homo sapiens
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview
Rattus norvegicus
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview
Saccharomyces cerevisiae
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Homo sapiens
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Rattus norvegicus
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Saccharomyces cerevisiae
Other publictions for EC 1.3.1.93
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744944
Lehka
Improving heterologous produc ...
Saccharomyces cerevisiae
FEMS Yeast Res.
17
doi: 10.1093/femsyr/fox004
2017
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2
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745307
Wakashima
Dual functions of the trans-2 ...
Homo sapiens, Rattus norvegicus, Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
J. Biol. Chem.
289
24736-24748
2014
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3
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699263
Song
Characterization of two cotton ...
Gossypium hirsutum
J. Exp. Bot.
60
1839-1848
2009
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4
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1
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2
2
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719840
Paul
A six-membrane-spanning topolo ...
Arabidopsis thaliana, Saccharomyces cerevisiae
J. Biol. Chem.
282
19237-19246
2007
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11
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720410
Kvam
Targeting of Tsc13p to nucleus ...
Saccharomyces cerevisiae
Mol. Biol. Cell
16
3987-3998
2005
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720671
Zheng
Disruptions of the Arabidopsis ...
Arabidopsis thaliana
Plant Cell
17
1467-1481
2005
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720124
Gable
Functional characterization of ...
Arabidopsis thaliana
J. Exp. Bot.
55
543-545
2004
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4
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1
1
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720450
Kohlwein
Tsc13p is required for fatty a ...
Saccharomyces cerevisiae
Mol. Cell. Biol.
21
109-125
2001
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