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Literature summary for 1.3.1.91 extracted from

  • Faivre, B.; Lombard, M.; Fakroun, S.; Vo, C.; Goyenvalle, C.; Guerineau, V.; Pecqueur, L.; Fontecave, M.; De Crecy-Lagard, V.; Bregeon, D.; Hamdane, D.
    Dihydrouridine synthesis in tRNAs is under reductive evolution in Mollicutes (2021), RNA Biol., 18, 2278-2289 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21DE3 Star cells Mycoplasma capricolum subsp. capricolum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil17 in tRNA + NADPH + H+ Mycoplasma capricolum subsp. capricolum
-
5,6-dihydrouracil17 in tRNA + NADP+
-
?
uracil17 in tRNA + NADPH + H+ Mycoplasma capricolum subsp. capricolum ATCC 14323
-
5,6-dihydrouracil17 in tRNA + NADP+
-
?
uracil20 in tRNA + NADPH + H+ Mycoplasma capricolum subsp. capricolum
-
5,6-dihydrouracil20 in tRNA + NADP+
-
?
uracil20 in tRNA + NADPH + H+ Mycoplasma capricolum subsp. capricolum ATCC 14323
-
5,6-dihydrouracil20 in tRNA + NADP+
-
?
uracil20a in tRNA + NADPH + H+ Mycoplasma capricolum subsp. capricolum
-
5,6-dihydrouracil20a in tRNA + NADP+
-
?
uracil20a in tRNA + NADPH + H+ Mycoplasma capricolum subsp. capricolum ATCC 14323
-
5,6-dihydrouracil20a in tRNA + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Mycoplasma capricolum subsp. capricolum A0A084EMY4
-
-
Mycoplasma capricolum subsp. capricolum ATCC 14323 A0A084EMY4
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography and Superdex S200 gel filtration Mycoplasma capricolum subsp. capricolum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
more the enzyme it is not able to synthetize uracil16 in tRNA
-
Mycoplasma capricolum subsp. capricolum ?
-
?
more the enzyme it is not able to synthetize uracil16 in tRNA
-
Mycoplasma capricolum subsp. capricolum ATCC 14323 ?
-
?
uracil in bulk tRNA + NADH + H+ the enzyme exhibits a clear preference for NADPH compared to NADH Mycoplasma capricolum subsp. capricolum 5,6-dihydrouracil in bulk tRNA + NAD+
-
?
uracil in bulk tRNA + NADH + H+ the enzyme exhibits a clear preference for NADPH compared to NADH Mycoplasma capricolum subsp. capricolum ATCC 14323 5,6-dihydrouracil in bulk tRNA + NAD+
-
?
uracil in bulk tRNA + NADPH + H+ the enzyme exhibits a clear preference for NADPH compared to NADH Mycoplasma capricolum subsp. capricolum 5,6-dihydrouracil in bulk tRNA + NADP+
-
?
uracil17 in tRNA + NADPH + H+
-
Mycoplasma capricolum subsp. capricolum 5,6-dihydrouracil17 in tRNA + NADP+
-
?
uracil17 in tRNA + NADPH + H+
-
Mycoplasma capricolum subsp. capricolum ATCC 14323 5,6-dihydrouracil17 in tRNA + NADP+
-
?
uracil20 in tRNA + NADPH + H+
-
Mycoplasma capricolum subsp. capricolum 5,6-dihydrouracil20 in tRNA + NADP+
-
?
uracil20 in tRNA + NADPH + H+
-
Mycoplasma capricolum subsp. capricolum ATCC 14323 5,6-dihydrouracil20 in tRNA + NADP+
-
?
uracil20a in tRNA + NADPH + H+
-
Mycoplasma capricolum subsp. capricolum 5,6-dihydrouracil20a in tRNA + NADP+
-
?
uracil20a in tRNA + NADPH + H+
-
Mycoplasma capricolum subsp. capricolum ATCC 14323 5,6-dihydrouracil20a in tRNA + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Mycoplasma capricolum subsp. capricolum

Synonyms

Synonyms Comment Organism
dihydrouridine synthase
-
Mycoplasma capricolum subsp. capricolum
DusB
-
Mycoplasma capricolum subsp. capricolum

Cofactor

Cofactor Comment Organism Structure
NADH
-
Mycoplasma capricolum subsp. capricolum
NADPH most effective cofactor Mycoplasma capricolum subsp. capricolum