Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. There is a higher proportion of tRNAGly in Escherichia coli expressinf DUS2 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C117A | rate constant is 1600fold lower than the rate constant of the wild-type enzyme | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P53720 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil in tRNA + NAD(P)H + H+ | the enzyme is specific for the proR hydrogen of NADPH. Cys117 is very important for the reduction of tRNA | Saccharomyces cerevisiae | 5,6-dihydrouracil in tRNA + NAD(P)+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
dihydrouridine synthase 2 | - |
Saccharomyces cerevisiae |
DUS 2 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of reductive and oxidative half-reactions | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavin-dependent enzyme | Saccharomyces cerevisiae |