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Literature summary for 1.3.1.9 extracted from
- Crystal structures and kinetic properties of enoyl-acyl carrier protein reductase I from Candidatus Liberibacter asiaticus (2014), Protein Sci., 23, 366-377.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Candidatus Liberibacter asiaticus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| apoform and in complex with NAD+, hanging drop vapor diffusion method, using | Candidatus Liberibacter asiaticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| isoniazid | competitive inhibitor with respect to NADH | Candidatus Liberibacter asiaticus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0175 | - |
crotonyl-CoA | at pH 7.5 and 28°C | Candidatus Liberibacter asiaticus | |
| 0.0205 | - |
NADH | at pH 7.5 and 28°C | Candidatus Liberibacter asiaticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61600 | - |
gel filtration | Candidatus Liberibacter asiaticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candidatus Liberibacter asiaticus | C6XEY3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ Hi-Trap column chromatography and Superdex 200 gel filtration | Candidatus Liberibacter asiaticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| crotonyl-CoA + NADH + H+ | - |
Candidatus Liberibacter asiaticus | butyryl-CoA + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | x-ray crystallography | Candidatus Liberibacter asiaticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enoyl-acyl carrier protein reductase I | - |
Candidatus Liberibacter asiaticus |
| ENR | - |
Candidatus Liberibacter asiaticus |
| FabI | - |
Candidatus Liberibacter asiaticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Candidatus Liberibacter asiaticus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00011 | - |
isoniazid | with respect to NADH, at pH 7.5 and 28°C | Candidatus Liberibacter asiaticus | |
| 0.0068 | - |
isoniazid | with respect to crotonyl-CoA, at pH 7.5 and 28°C | Candidatus Liberibacter asiaticus | |
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Release 2023.1 (January 2023)
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