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Literature summary for 1.3.1.9 extracted from

  • Vick, J.E.; Clomburg, J.M.; Blankschien, M.D.; Chou, A.; Kim, S.; Gonzalez, R.
    Escherichia coli enoyl-acyl carrier protein reductase (FabI) supports efficient operation of a functional reversal of ?-oxidation cycle (2015), Appl. Environ. Microbiol., 81, 1406-1416.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.9

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA about 20% inhibition at 0.2 mM Escherichia coli
decanoyl-CoA about 15% inhibition at 0.2 mM Escherichia coli
lauroyl-CoA about 50% inhibition at 0.2 mM Escherichia coli
additional information not inhibited by 3-hydroxybutyryl-CoA, butyryl-CoA, hexanoyl-CoA, octanoyl-CoA Escherichia coli
myristoyl-CoA about 80% inhibition at 0.2 mM Escherichia coli
palmitic acid
-
 Escherichia coli
palmitoyl-CoA nearly 50% inhibition at 0.02 mM Escherichia coli
stearoyl-CoA about 40% inhibition at 0.2 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6
-
 crotonyl-CoA at pH 7.5 and 30°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ has marginal effects on activity level Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an acyl-[acyl-carrier protein] + NAD+ Escherichia coli
-
 a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon metal affinity resin column chromatography, and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an acyl-[acyl-carrier protein] + NAD+
-
 Escherichia coli a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?
crotonyl-CoA + NADH + H+
-
 Escherichia coli butyryl-CoA + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
enoyl-acyl carrier protein reductase
-
 Escherichia coli
ENR
-
 Escherichia coli
FabI
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3
-
 crotonyl-CoA at pH 7.5 and 30°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Escherichia coli
NADH
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0054
-
 palmitic acid at pH 7.5 and 30°C Escherichia coli
0.9
-
 decanoyl-CoA at pH 7.5 and 30°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.15
-
 crotonyl-CoA at pH 7.5 and 30°C Escherichia coli