Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
trans-but-2-enoyl-[acyl-carrier protein] | 30°C, pH 6.5, in the presence of 100 mM NH4+ | Streptococcus pneumoniae | |
0.023 | - |
NADH | 30°C, pH 6.5, in the presence of 100 mM NH4+ | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ | Streptococcus pneumoniae | |
K+ | activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ | Streptococcus pneumoniae | |
Na+ | activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ | Streptococcus pneumoniae | |
NH4+ | 15fold activation at 100 mM | Streptococcus pneumoniae | |
Rb+ | activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant FabK | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-but-2-enoyl-[acyl-carrier protein] + NADH | highly selective for NADH | Streptococcus pneumoniae | butanoyl-[acyl-carrrier-protein] + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FabK | - |
Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
trans-but-2-enoyl-[acyl-carrier protein] | 30°C, pH 6.5, in the absence of 100 mM NH4+ | Streptococcus pneumoniae | |
70 | - |
trans-but-2-enoyl-[acyl-carrier protein] | 30°C, pH 6.5, in the presence of 100 mM NH4+ | Streptococcus pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Streptococcus pneumoniae | |
NADH | - |
Streptococcus pneumoniae |