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Literature summary for 1.3.1.9 extracted from
- Studies on the mechanism of fatty acid synthesis. 18. Preparation and general properties of the enoyl acyl carrier protein reductases from Escherichia coli (1968), J. Biol. Chem., 243, 1180-1189.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | stimulation | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-hydroxymercuribenzoate | - |
Escherichia coli | |
| iodoacetate | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
crotonyl-[acyl-carrier protein] | - |
Escherichia coli | |
| 2.5 | - |
crotonyl-CoA | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| no separation from NADPH-specific enzyme | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hexenoyl-[acyl-carrier protein] + NADH | - |
Escherichia coli | hexanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
| 2-octenoyl-[acyl-carrier protein] + NADH | - |
Escherichia coli | octanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
| crotonyl-[acyl-carrier protein] + NADH | - |
Escherichia coli | butyryl-[acyl-carrier protein] + NAD+ | - |
ir | |
| additional information | also active on enoyl-CoA substrates | Escherichia coli | ? | - |
? | |
| additional information | substrate specificity: acyl chain length C4-C16 | Escherichia coli | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
5 min stable | Escherichia coli |
| 60 | - |
5 min, inactivation | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
relatively stable above | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | absolute specificity | Escherichia coli | |
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