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Literature summary for 1.3.1.87 extracted from

  • Diaz, E.; Ferrandez, A.; Garcia, J.L.
    Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12 (1998), J. Bacteriol., 180, 2915-2923.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28500
-
calculated from amino acid sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephadex G-25M gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phenylpropionate + NAD+
-
Escherichia coli 3-(2,3-dihydroxyphenyl) propionate + NADH + H+
-
?
cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + NAD+
-
Escherichia coli ? + NADH + H+
-
ir
cis-3-(2-carboxyethyl)-3,5-cyclohexadien-1,2-diol + NAD+
-
Escherichia coli 3-(2,3-dihydroxyphenyl)propionate + NADH + H+
-
ir

Synonyms

Synonyms Comment Organism
2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase
-
Escherichia coli
3-phenylpropionate-2',3'-dihydrodiol dehydrogenase
-
Escherichia coli
3-phenylpropionate-dihydrodiol dehydrogenase
-
Escherichia coli
HcaB
-
Escherichia coli
HCAR HcaR controls expression of the neighbouring genes, hcaA1, hcaA2, hcaC, hcaB and hcaD, coding for the 3-phenylpropionate dioxygenase complex and 3-phenylpropionate-2',3'-dihydrodiol dehydrogenase Escherichia coli

Expression

Organism Comment Expression
Escherichia coli when bacteria are grown in the absence of oxygen, the expression of hcaR is decreased threefold compared to growth in aerobic conditions down