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Literature summary for 1.3.1.86 extracted from

  • Aboulnaga, e.l.-.H.; Pinkenburg, O.; Schiffels, J.; El-Refai, A.; Buckel, W.; Selmer, T.
    Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli (2013), J. Bacteriol., 195, 3704-3713.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.86

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Clostridioides difficile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 Ferredoxin pH 7.5, temperature not specified in the publication Clostridioides difficile
0.0025
-
 crotonyl-CoA pH 7.5, temperature not specified in the publication Clostridioides difficile
0.01
-
 butyryl-CoA pH 7.5, temperature not specified in the publication Clostridioides difficile
0.145
-
 NADH pH 7.5, temperature not specified in the publication Clostridioides difficile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
-
 Clostridioides difficile

Organism

Organism UniProt Comment Textmining
Clostridioides difficile
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + NADH + ferredoxin
-
 Clostridioides difficile butyryl-CoA + ?
-
 ?

Synonyms

Synonyms Comment Organism
bcd2
-
 Clostridioides difficile
butyryl-CoA dehydrogenase complex
-
 Clostridioides difficile
CD1054
-
 Clostridioides difficile

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 butyryl-CoA pH 7.5, temperature not specified in the publication Clostridioides difficile
3 6 NADH pH 7.5, temperature not specified in the publication Clostridioides difficile
19
-
 crotonyl-CoA pH 7.5, temperature not specified in the publication Clostridioides difficile
37
-
 Ferredoxin pH 7.5, temperature not specified in the publication Clostridioides difficile

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Clostridioides difficile

Cofactor

Cofactor Comment Organism Structure
FAD in the absence of ferredoxin, a remarkable stimulation of crotonyl-CoA reduction by FAD is observed Clostridioides difficile
Ferredoxin butyryl-CoA dehydrogenase from C. difficile belongs to the subfamily of bifurcating enzymes capable of coupling the exergonic reduction of crotonyl-CoA by NAD Hwith the endergonic reduction of ferredoxin by NADH Clostridioides difficile
NADH
-
 Clostridioides difficile

General Information

General Information Comment Organism
metabolism butyryl-CoA dehydrogenase from C. difficile belongs to the subfamily of bifurcating enzymes capable of coupling the exergonic reduction of crotonyl-CoA by NAD Hwith the endergonic reduction of ferredoxin by NADH Clostridioides difficile
metabolism the genes necessary for butyrate formation from the genome of Clostridium difficile are expressed in Escherichia coli. The individual genes are assembled in a single plasmid vector into an artificial operon , which allows functional coexpression of the required genes and confers butyrate-forming capability to the host Clostridioides difficile

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
250
-
 NADH pH 7.5, temperature not specified in the publication Clostridioides difficile
2800
-
 butyryl-CoA pH 7.5, temperature not specified in the publication Clostridioides difficile
7600
-
 crotonyl-CoA pH 7.5, temperature not specified in the publication Clostridioides difficile
18500
-
 Ferredoxin pH 7.5, temperature not specified in the publication Clostridioides difficile