BRENDA - Enzyme Database
show all sequences of 1.3.1.85

A crotonyl-CoA reductase-carboxylase independent pathway for assembly of unusual alkylmalonyl-CoA polyketide synthase extender units

Ray, L.; Valentic, T.R.; Miyazawa, T.; Withall, D.M.; Song, L.; Milligan, J.C.; Osada, H.; Takahashi, S.; Tsai, S.C.; Challis, G.L.; Nat. Commun. 7, 13609 (2016)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
crotonyl-CoA + CO2 + NADPH + H+
Streptomyces ambofaciens
-
?
-
-
?
crotonyl-CoA + CO2 + NADPH + H+
Streptomyces ambofaciens ATCC23877
-
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces ambofaciens
-
-
-
Streptomyces ambofaciens ATCC23877
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E)-oct-2-enoyl-CoA + NADPH + CO2 + H+
-
741053
Streptomyces ambofaciens
(2S)-2-carboxyoctanoyl-CoA + NADP+
-
-
-
?
(2E)-oct-2-enoyl-CoA + NADPH + CO2 + H+
-
741053
Streptomyces ambofaciens ATCC23877
(2S)-2-carboxyoctanoyl-CoA + NADP+
-
-
-
?
crotonyl-CoA + CO2 + NADPH + H+
-
741053
Streptomyces ambofaciens
?
-
-
-
?
crotonyl-CoA + CO2 + NADPH + H+
-
741053
Streptomyces ambofaciens ATCC23877
?
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Streptomyces ambofaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Streptomyces ambofaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
crotonyl-CoA + CO2 + NADPH + H+
Streptomyces ambofaciens
-
?
-
-
?
crotonyl-CoA + CO2 + NADPH + H+
Streptomyces ambofaciens ATCC23877
-
?
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E)-oct-2-enoyl-CoA + NADPH + CO2 + H+
-
741053
Streptomyces ambofaciens
(2S)-2-carboxyoctanoyl-CoA + NADP+
-
-
-
?
(2E)-oct-2-enoyl-CoA + NADPH + CO2 + H+
-
741053
Streptomyces ambofaciens ATCC23877
(2S)-2-carboxyoctanoyl-CoA + NADP+
-
-
-
?
crotonyl-CoA + CO2 + NADPH + H+
-
741053
Streptomyces ambofaciens
?
-
-
-
?
crotonyl-CoA + CO2 + NADPH + H+
-
741053
Streptomyces ambofaciens ATCC23877
?
-
-
-
?
Other publictions for EC 1.3.1.85
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741053
Ray
A crotonyl-CoA reductase-carbo ...
Streptomyces ambofaciens, Streptomyces ambofaciens ATCC23877
Nat. Commun.
7
13609
2016
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2
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2
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1
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4
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739852
Zhang
Rational control of polyketide ...
Streptomyces sp., Streptomyces sp. NRRL 2288
Angew. Chem. Int. Ed. Engl.
54
13462-13465
2015
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5
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9
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4
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8
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9
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1
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5
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9
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8
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9
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8
8
740962
Blazic
Roles of the crotonyl-CoA carb ...
Streptomyces tsukubensis, Streptomyces tsukubensis NRRL 18488
Microb. Cell Fact.
14
164
2015
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2
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3
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2
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741243
Escudero
New rimocidin/CE-108 derivativ ...
Streptomyces diastaticus
PLoS ONE
10
e0135891
2015
-
-
1
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1
-
2
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1
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1
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1
1
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1
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1
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739814
Zhang
Crystallization and preliminar ...
Streptomyces sp., Streptomyces sp. NRRL 2288
Acta Crystallogr. Sect. F
70
734-737
2014
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-
1
1
-
-
-
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2
2
-
5
-
-
1
-
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-
14
1
-
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-
-
1
-
-
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-
1
1
1
-
-
-
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-
2
2
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-
1
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-
14
1
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725270
Schneider
Rhodobacter sphaeroides uses a ...
Rhodobacter sphaeroides
J. Bacteriol.
194
225-232
2012
1
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4
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1
1
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-
725271
Hu
CcrR, a TetR family transcript ...
Methylorubrum extorquens, Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
J. Bacteriol.
194
2802-2808
2012
-
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16
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1
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1
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724588
Yoo
Characterization of 2-octenoyl ...
Streptomyces avermitilis
Biosci. Biotechnol. Biochem.
75
1191-1193
2011
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1
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2
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4
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1
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1
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1
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4
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1
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1
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710982
Alber
Biotechnological potential of ...
Methylorubrum extorquens, Rhodobacter sphaeroides, Streptomyces coelicolor
Appl. Microbiol. Biotechnol.
89
17-25
2010
-
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6
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6
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6
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6
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6
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3
3
3
3
-
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713409
Erb
Carboxylation mechanism and st ...
Rhodobacter sphaeroides
Proc. Natl. Acad. Sci. USA
106
8871-8876
2009
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5
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2
1
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1
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1
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4
1
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1
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1
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1
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4
1
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1
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689745
Erb
Synthesis of C5-dicarboxylic a ...
Methylorubrum extorquens AM1, Rhodobacter sphaeroides 2.4.1, Streptomyces coelicolor
Proc. Natl. Acad. Sci. USA
104
10631-10636
2007
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1
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4
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2
2
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8
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1
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3
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6
1
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1
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3
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1
3
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4
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2
2
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1
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3
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6
1
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1
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3
3
-
1
1