Literature summary for 1.3.1.85 extracted from

Zhang, L.; Mori, T.; Zheng, Q.; Awakawa, T.; Yan, Y.; Liu, W.; Abe, I.
Rational control of polyketide extender units by structure-based engineering of a crotonyl-CoA carboxylase/reductase in antimycin biosynthesis (2015), Angew. Chem. Int. Ed. Engl., 54, 13462-13465.

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Protein Variants

Protein Variants	Comment	Organism
A182G	inactive	Streptomyces sp.
A182L	the mutant with increased activity selectively catalyzes carboxylation over the regular reduction reaction	Streptomyces sp.
V350A	the mutant shows increased activity compared to the wild type enzyme	Streptomyces sp.
V350F	the mutant shows increased activity compared to the wild type enzyme	Streptomyces sp.
V350G	the mutant shows increased activity compared to the wild type enzyme	Streptomyces sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.089	-	cinnamoyl-CoA	mutant enzyme V350G, pH and temperature not specified in the publication	Streptomyces sp.
0.14	-	4-coumaroyl-CoA	mutant enzyme V350A, pH and temperature not specified in the publication	Streptomyces sp.
0.2	-	4-coumaroyl-CoA	mutant enzyme V350G, pH and temperature not specified in the publication	Streptomyces sp.
0.38	-	cinnamoyl-CoA	wild type enzyme, pH and temperature not specified in the publication	Streptomyces sp.
0.49	-	crotonyl-CoA	mutant enzyme V350F, pH and temperature not specified in the publication	Streptomyces sp.
1.17	-	crotonyl-CoA	mutant enzyme V350G, pH and temperature not specified in the publication	Streptomyces sp.
1.21	-	crotonyl-CoA	mutant enzyme V350A, pH and temperature not specified in the publication	Streptomyces sp.
1.31	-	crotonyl-CoA	mutant enzyme A182L, pH and temperature not specified in the publication	Streptomyces sp.
1.58	-	crotonyl-CoA	wild type enzyme, pH and temperature not specified in the publication	Streptomyces sp.

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp.	M1SQA1	-	-
Streptomyces sp. NRRL 2288	M1SQA1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-indolylacryloyl-CoA + NADPH + CO2 + H+	-	Streptomyces sp.	indolylmethylmalonyl-CoA + NADP+	-	?
3-indolylacryloyl-CoA + NADPH + CO2 + H+	-	Streptomyces sp. NRRL 2288	indolylmethylmalonyl-CoA + NADP+	-	?
4-coumaroyl-CoA + NADPH + CO2 + H+	-	Streptomyces sp.	(2S)2-carboxy-3-(4-hydroxyphenyl)propionyl-CoA + NADP+	-	?
4-coumaroyl-CoA + NADPH + CO2 + H+	-	Streptomyces sp. NRRL 2288	(2S)2-carboxy-3-(4-hydroxyphenyl)propionyl-CoA + NADP+	-	?
cinnamoyl-CoA + NADPH + CO2 + H+	-	Streptomyces sp.	(2S)2-carboxy-3-phenylpropionyl-CoA + NADP+	-	?
cinnamoyl-CoA + NADPH + CO2 + H+	-	Streptomyces sp. NRRL 2288	(2S)2-carboxy-3-phenylpropionyl-CoA + NADP+	-	?
crotonyl-CoA + NADPH + CO2 + H+	-	Streptomyces sp.	(2S)-ethylmalonyl-CoA + NADP+	-	?
crotonyl-CoA + NADPH + CO2 + H+	-	Streptomyces sp. NRRL 2288	(2S)-ethylmalonyl-CoA + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
AntE	-	Streptomyces sp.
CCR	-	Streptomyces sp.
crotonyl-CoA carboxylase/reductase	-	Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0675	-	crotonyl-CoA	mutant enzyme V350G, pH and temperature not specified in the publication	Streptomyces sp.
0.0688	-	cinnamoyl-CoA	wild type enzyme, pH and temperature not specified in the publication	Streptomyces sp.
0.0735	-	4-coumaroyl-CoA	mutant enzyme V350A, pH and temperature not specified in the publication	Streptomyces sp.
0.0877	-	cinnamoyl-CoA	mutant enzyme V350G, pH and temperature not specified in the publication	Streptomyces sp.
0.109	-	4-coumaroyl-CoA	mutant enzyme V350G, pH and temperature not specified in the publication	Streptomyces sp.
0.1197	-	crotonyl-CoA	mutant enzyme V350A, pH and temperature not specified in the publication	Streptomyces sp.
0.1263	-	crotonyl-CoA	mutant enzyme V350F, pH and temperature not specified in the publication	Streptomyces sp.
0.1519	-	crotonyl-CoA	mutant enzyme A182L, pH and temperature not specified in the publication	Streptomyces sp.
0.1755	-	crotonyl-CoA	wild type enzyme, pH and temperature not specified in the publication	Streptomyces sp.

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Streptomyces sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0583	-	crotonyl-CoA	mutant enzyme V350G, pH and temperature not specified in the publication	Streptomyces sp.
0.111	-	crotonyl-CoA	wild type enzyme, pH and temperature not specified in the publication	Streptomyces sp.
0.115	-	crotonyl-CoA	mutant enzyme A182L, pH and temperature not specified in the publication	Streptomyces sp.
0.18	-	cinnamoyl-CoA	wild type enzyme, pH and temperature not specified in the publication	Streptomyces sp.
0.257	-	crotonyl-CoA	mutant enzyme V350A, pH and temperature not specified in the publication	Streptomyces sp.
0.257	-	crotonyl-CoA	mutant enzyme V350F, pH and temperature not specified in the publication	Streptomyces sp.
0.525	-	4-coumaroyl-CoA	mutant enzyme V350A, pH and temperature not specified in the publication	Streptomyces sp.
0.545	-	4-coumaroyl-CoA	mutant enzyme V350G, pH and temperature not specified in the publication	Streptomyces sp.
0.985	-	cinnamoyl-CoA	mutant enzyme V350G, pH and temperature not specified in the publication	Streptomyces sp.

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Release 2023.1 (January 2023)