BRENDA - Enzyme Database
show all sequences of 1.3.1.85

Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase

Erb, T.J.; Brecht, V.; Fuchs, G.; Muller, M.; Alber, B.E.; Proc. Natl. Acad. Sci. USA 106, 8871-8876 (2009)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
CO2
at pH 7.8, temperature not specified in the publication
Rhodobacter sphaeroides
0.2
-
(E)-crotonyl-CoA
for reduction, pH and temperature not specified in the publication
Rhodobacter sphaeroides
0.4
-
(E)-crotonyl-CoA
for reductive carboxylation, pH and temperature not specified in the publication
Rhodobacter sphaeroides
0.5
-
acryloyl-CoA
pH and temperature not specified in the publication
Rhodobacter sphaeroides
0.7
-
NADPH
pH and temperature not specified in the publication
Rhodobacter sphaeroides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
2 * 47000, gel filtration
Rhodobacter sphaeroides
105000
-
gel filtration
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(E)-crotonyl-CoA + CO2 + NADPH + H+
Rhodobacter sphaeroides
100% relative specific activity, the carboxylation reaction is the physiologically relevant reaction
(2S)-ethylmalonyl-CoA + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodobacter sphaeroides
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rhodobacter sphaeroides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-crotonyl-CoA + CO2 + NADPH + H+
100% relative specific activity, the carboxylation reaction is the physiologically relevant reaction
713409
Rhodobacter sphaeroides
(2S)-ethylmalonyl-CoA + NADP+
-
-
-
r
(E)-crotonyl-CoA + NADPH + H+
10% of maximal activity (compared with vmax of crotonyl-CoA carboxylation)
713409
Rhodobacter sphaeroides
butyryl-CoA + NADP+
-
-
-
?
acryloyl-CoA + CO2 + NADPH + H+
acryloyl-CoA is accepted as an alternative substrate analogue by the enzyme with 40% relative activity (compared with vmax of crotonyl-CoA carboxylation)
713409
Rhodobacter sphaeroides
methylmalonyl-CoA + NADP+
-
-
-
?
additional information
does not reduce crotonyl-N-acetylcysteamine, methacryloyl-CoA, 6-hydroxycylohex-1-ene-1-carboxyl-CoA, cyclo-hexa-1,5-diene-1-carboxyl-CoA, acetoacetyl-CoA, propionyl-CoA, (R)-3-hydroxybutyryl-CoA, (S)-3-hydroxybutyryl-CoA (less than 1% specific activity)
713409
Rhodobacter sphaeroides
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 47000, gel filtration
Rhodobacter sphaeroides
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Rhodobacter sphaeroides
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Rhodobacter sphaeroides
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Rhodobacter sphaeroides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
CO2
at pH 7.8, temperature not specified in the publication
Rhodobacter sphaeroides
0.2
-
(E)-crotonyl-CoA
for reduction, pH and temperature not specified in the publication
Rhodobacter sphaeroides
0.4
-
(E)-crotonyl-CoA
for reductive carboxylation, pH and temperature not specified in the publication
Rhodobacter sphaeroides
0.5
-
acryloyl-CoA
pH and temperature not specified in the publication
Rhodobacter sphaeroides
0.7
-
NADPH
pH and temperature not specified in the publication
Rhodobacter sphaeroides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
2 * 47000, gel filtration
Rhodobacter sphaeroides
105000
-
gel filtration
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(E)-crotonyl-CoA + CO2 + NADPH + H+
Rhodobacter sphaeroides
100% relative specific activity, the carboxylation reaction is the physiologically relevant reaction
(2S)-ethylmalonyl-CoA + NADP+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rhodobacter sphaeroides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-crotonyl-CoA + CO2 + NADPH + H+
100% relative specific activity, the carboxylation reaction is the physiologically relevant reaction
713409
Rhodobacter sphaeroides
(2S)-ethylmalonyl-CoA + NADP+
-
-
-
r
(E)-crotonyl-CoA + NADPH + H+
10% of maximal activity (compared with vmax of crotonyl-CoA carboxylation)
713409
Rhodobacter sphaeroides
butyryl-CoA + NADP+
-
-
-
?
acryloyl-CoA + CO2 + NADPH + H+
acryloyl-CoA is accepted as an alternative substrate analogue by the enzyme with 40% relative activity (compared with vmax of crotonyl-CoA carboxylation)
713409
Rhodobacter sphaeroides
methylmalonyl-CoA + NADP+
-
-
-
?
additional information
does not reduce crotonyl-N-acetylcysteamine, methacryloyl-CoA, 6-hydroxycylohex-1-ene-1-carboxyl-CoA, cyclo-hexa-1,5-diene-1-carboxyl-CoA, acetoacetyl-CoA, propionyl-CoA, (R)-3-hydroxybutyryl-CoA, (S)-3-hydroxybutyryl-CoA (less than 1% specific activity)
713409
Rhodobacter sphaeroides
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 47000, gel filtration
Rhodobacter sphaeroides
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Rhodobacter sphaeroides
Other publictions for EC 1.3.1.85
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741053
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A crotonyl-CoA reductase-carbo ...
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1
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741243
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2
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1
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70
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1
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2
2
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1
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14
1
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1
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1
1
1
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2
2
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1
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14
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725270
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194
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16
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1
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1
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724588
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4
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1
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1
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710982
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2010
-
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6
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6
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6
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6
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6
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3
3
3
3
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713409
Erb
Carboxylation mechanism and st ...
Rhodobacter sphaeroides
Proc. Natl. Acad. Sci. USA
106
8871-8876
2009
-
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5
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2
1
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1
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1
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4
1
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1
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1
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1
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5
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1
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4
1
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1
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689745
Erb
Synthesis of C5-dicarboxylic a ...
Methylorubrum extorquens AM1, Rhodobacter sphaeroides 2.4.1, Streptomyces coelicolor
Proc. Natl. Acad. Sci. USA
104
10631-10636
2007
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1
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4
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2
2
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8
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1
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3
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6
1
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3
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1
3
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2
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1
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3
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6
1
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1
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3
3
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1
1