BRENDA - Enzyme Database
show all sequences of 1.3.1.85

Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway

Erb, T.J.; Berg, I.A.; Brecht, V.; Mueller, M.; Fuchs, G.; Alber, B.E.; Proc. Natl. Acad. Sci. USA 104, 10631-10636 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Rhodobacter sphaeroides 2.4.1
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
(2S)-ethylmalonyl-CoA
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
0.2
-
CO2
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
0.4
-
crotonyl-CoA
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
0.7
-
NADPH
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
comprehensive metal analysis of crotonyl-CoA carboxylase/reductase by plasma emission spectroscopy indicates the absence of any metals
Methylorubrum extorquens AM1
additional information
comprehensive metal analysis of crotonyl-CoA carboxylase/reductase by plasma emission spectroscopy indicates the absence of any metals
Rhodobacter sphaeroides 2.4.1
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47700
-
2 * 47700, gel filtration
Rhodobacter sphaeroides 2.4.1
105000
-
gel filtration
Rhodobacter sphaeroides 2.4.1
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methylorubrum extorquens AM1
-
-
-
Rhodobacter sphaeroides 2.4.1
-
-
-
Streptomyces coelicolor
-
strain A3
-
Purification (Commentary)
Commentary
Organism
DEAE-Sepharose column chromatography and Cibacron Blue 3GA agarose column chromatography
Rhodobacter sphaeroides 2.4.1
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:acetate-grown cell
-
Rhodobacter sphaeroides 2.4.1
-
culture condition:butyrate-grown cell
-
Streptomyces coelicolor
-
culture condition:methanol-grown cell
-
Methylorubrum extorquens AM1
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
crotonyl-CoA + CO2 + NADPH + H+
crotonyl-CoA is the best substrate
689745
Streptomyces coelicolor
(2S)-ethylmalonyl-CoA + NADP+
-
-
-
r
crotonyl-CoA + CO2 + NADPH + H+
crotonyl-CoA is the best substrate
689745
Methylorubrum extorquens AM1
(2S)-ethylmalonyl-CoA + NADP+
-
-
-
r
crotonyl-CoA + CO2 + NADPH + H+
crotonyl-CoA is the best substrate
689745
Rhodobacter sphaeroides 2.4.1
(2S)-ethylmalonyl-CoA + NADP+
-
-
-
r
crotonyl-CoA + NADPH + H+
reduction at low rate
689745
Streptomyces coelicolor
butyryl-CoA + NADP+
-
-
-
ir
crotonyl-CoA + NADPH + H+
reduction at low rate
689745
Methylorubrum extorquens AM1
butyryl-CoA + NADP+
-
-
-
ir
crotonyl-CoA + NADPH + H+
reduction at low rate
689745
Rhodobacter sphaeroides 2.4.1
butyryl-CoA + NADP+
-
-
-
ir
Subunits
Subunits
Commentary
Organism
homodimer
2 * 47700, gel filtration
Rhodobacter sphaeroides 2.4.1
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
104
-
(2S)-ethylmalonyl-CoA
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Methylorubrum extorquens AM1
NADPH
-
Rhodobacter sphaeroides 2.4.1
NADPH
-
Streptomyces coelicolor
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Rhodobacter sphaeroides 2.4.1
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Methylorubrum extorquens AM1
NADPH
-
Rhodobacter sphaeroides 2.4.1
NADPH
-
Streptomyces coelicolor
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
(2S)-ethylmalonyl-CoA
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
0.2
-
CO2
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
0.4
-
crotonyl-CoA
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
0.7
-
NADPH
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
comprehensive metal analysis of crotonyl-CoA carboxylase/reductase by plasma emission spectroscopy indicates the absence of any metals
Methylorubrum extorquens AM1
additional information
comprehensive metal analysis of crotonyl-CoA carboxylase/reductase by plasma emission spectroscopy indicates the absence of any metals
Rhodobacter sphaeroides 2.4.1
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47700
-
2 * 47700, gel filtration
Rhodobacter sphaeroides 2.4.1
105000
-
gel filtration
Rhodobacter sphaeroides 2.4.1
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Sepharose column chromatography and Cibacron Blue 3GA agarose column chromatography
Rhodobacter sphaeroides 2.4.1
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:acetate-grown cell
-
Rhodobacter sphaeroides 2.4.1
-
culture condition:butyrate-grown cell
-
Streptomyces coelicolor
-
culture condition:methanol-grown cell
-
Methylorubrum extorquens AM1
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
crotonyl-CoA + CO2 + NADPH + H+
crotonyl-CoA is the best substrate
689745
Streptomyces coelicolor
(2S)-ethylmalonyl-CoA + NADP+
-
-
-
r
crotonyl-CoA + CO2 + NADPH + H+
crotonyl-CoA is the best substrate
689745
Methylorubrum extorquens AM1
(2S)-ethylmalonyl-CoA + NADP+
-
-
-
r
crotonyl-CoA + CO2 + NADPH + H+
crotonyl-CoA is the best substrate
689745
Rhodobacter sphaeroides 2.4.1
(2S)-ethylmalonyl-CoA + NADP+
-
-
-
r
crotonyl-CoA + NADPH + H+
reduction at low rate
689745
Streptomyces coelicolor
butyryl-CoA + NADP+
-
-
-
ir
crotonyl-CoA + NADPH + H+
reduction at low rate
689745
Methylorubrum extorquens AM1
butyryl-CoA + NADP+
-
-
-
ir
crotonyl-CoA + NADPH + H+
reduction at low rate
689745
Rhodobacter sphaeroides 2.4.1
butyryl-CoA + NADP+
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 47700, gel filtration
Rhodobacter sphaeroides 2.4.1
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
104
-
(2S)-ethylmalonyl-CoA
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
General Information
General Information
Commentary
Organism
metabolism
crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway
Methylorubrum extorquens AM1
metabolism
crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway
Rhodobacter sphaeroides 2.4.1
metabolism
crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway
Streptomyces coelicolor
General Information (protein specific)
General Information
Commentary
Organism
metabolism
crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway
Methylorubrum extorquens AM1
metabolism
crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway
Rhodobacter sphaeroides 2.4.1
metabolism
crotonyl-CoA carboxylase/reductase is the key enzyme of the ethylmalonyl-CoA pathway
Streptomyces coelicolor
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
520
-
(2S)-ethylmalonyl-CoA
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
520
-
(2S)-ethylmalonyl-CoA
in 100 mM Tris-HCl buffer (pH 7.9), temperature not specified in the publication
Rhodobacter sphaeroides 2.4.1
Other publictions for EC 1.3.1.85
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741053
Ray
A crotonyl-CoA reductase-carbo ...
Streptomyces ambofaciens, Streptomyces ambofaciens ATCC23877
Nat. Commun.
7
13609
2016
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2
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2
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4
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1
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4
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739852
Zhang
Rational control of polyketide ...
Streptomyces sp., Streptomyces sp. NRRL 2288
Angew. Chem. Int. Ed. Engl.
54
13462-13465
2015
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5
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9
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4
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8
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9
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1
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5
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8
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9
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8
8
740962
Blazic
Roles of the crotonyl-CoA carb ...
Streptomyces tsukubensis, Streptomyces tsukubensis NRRL 18488
Microb. Cell Fact.
14
164
2015
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2
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3
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1
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2
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2
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741243
Escudero
New rimocidin/CE-108 derivativ ...
Streptomyces diastaticus
PLoS ONE
10
e0135891
2015
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-
1
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-
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1
-
2
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1
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1
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1
1
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1
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1
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739814
Zhang
Crystallization and preliminar ...
Streptomyces sp., Streptomyces sp. NRRL 2288
Acta Crystallogr. Sect. F
70
734-737
2014
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-
1
1
-
-
-
-
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-
2
2
-
5
-
-
1
-
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-
14
1
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-
1
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-
1
1
1
-
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2
2
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-
1
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14
1
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725270
Schneider
Rhodobacter sphaeroides uses a ...
Rhodobacter sphaeroides
J. Bacteriol.
194
225-232
2012
1
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4
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1
1
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725271
Hu
CcrR, a TetR family transcript ...
Methylorubrum extorquens, Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
J. Bacteriol.
194
2802-2808
2012
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16
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1
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1
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724588
Yoo
Characterization of 2-octenoyl ...
Streptomyces avermitilis
Biosci. Biotechnol. Biochem.
75
1191-1193
2011
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1
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4
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1
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1
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710982
Alber
Biotechnological potential of ...
Methylorubrum extorquens, Rhodobacter sphaeroides, Streptomyces coelicolor
Appl. Microbiol. Biotechnol.
89
17-25
2010
-
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6
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6
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6
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6
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6
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3
3
3
3
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713409
Erb
Carboxylation mechanism and st ...
Rhodobacter sphaeroides
Proc. Natl. Acad. Sci. USA
106
8871-8876
2009
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5
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2
1
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1
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1
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4
1
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1
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1
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1
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5
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1
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1
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4
1
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1
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689745
Erb
Synthesis of C5-dicarboxylic a ...
Methylorubrum extorquens AM1, Rhodobacter sphaeroides 2.4.1, Streptomyces coelicolor
Proc. Natl. Acad. Sci. USA
104
10631-10636
2007
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1
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4
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2
2
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8
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1
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3
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6
1
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1
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3
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1
3
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1
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3
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6
1
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1
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3
3
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1
1