Literature summary for 1.3.1.82 extracted from

  • Ringer, K.L.; McConkey, M.E.; Davis, E.M.; Rushing, G.W.; Croteau, R.
    Monoterpene double-bond reductases of the (-)-menthol biosynthetic pathway: isolation and characterization of cDNAs encoding (-)-isopiperitenone reductase and (+)-pulegone reductase of peppermint (2003), Arch. Biochem. Biophys., 418, 80-92.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, functional expression of wild-type enzyme in Escherichia coli strain BL21(DE3), expression of a mutant cloning artifact that has a seven residue deletion at the C-terminus and bores an additional 33 vector-derived residues Mentha x piperita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
(-)-isopiperitenone pH 5.5, recombinant enzyme Mentha x piperita
0.0022
-
NADPH pH 5.5, recombinant enzyme Mentha x piperita

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE Mentha x piperita
60000
-
about, gel filtration Mentha x piperita
34409
-
2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE Mentha x piperita

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-isopiperitenone + NADPH + H+ Mentha x piperita pathways of monoterpene biosynthesis, overview (+)-cis-isopulegone + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Mentha x piperita
-
cv. Black Mitcham
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type enzyme from Escherichia coli strain BL21(DE3) to over 60% homogeneity, and to over 90% purity for the truncated mutant enzyme Mentha x piperita

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Mentha x piperita
-
epidermis
-
Mentha x piperita
-
plant oil gland
-
Mentha x piperita
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-isopiperitenone + NADPH + H+ pathways of monoterpene biosynthesis, overview Mentha x piperita (+)-cis-isopulegone + NADP+
-
?
(-)-isopiperitenone + NADPH + H+ regiospecific and stereospecific reductase Mentha x piperita (+)-cis-isopulegone + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE Mentha x piperita

Synonyms

Synonyms Comment Organism
More (-)-isopiperitenone reductase is a member of the short-chain dehydrogenase/reductase superfamily Mentha x piperita

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
NADPH pH 5.5, recombinant enzyme Mentha x piperita
1.3
-
(-)-isopiperitenone pH 5.5, recombinant enzyme Mentha x piperita

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
truncated mutant enzyme Mentha x piperita
5.5
-
wild-type enzyme Mentha x piperita

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5 50% of maximal activity within this range, wild-type enzyme Mentha x piperita
5 8 50% of maximal activity within this range, deletion mutant enzyme Mentha x piperita

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Mentha x piperita