Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain BL21(DE3) | Mentha x piperita |
General Stability | Organism |
---|---|
neither protease inhibitors nor flavins improve the activity or stability of the purified native enzyme, and the addition of glycerol to the buffers leads to rapid loss of activity upon storage | Mentha x piperita |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
(+)-pulegone | pH 5.0, recombinant enzyme | Mentha x piperita | |
0.0069 | - |
NADPH | pH 5.0, recombinant enzyme | Mentha x piperita |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37914 | - |
1 x * 43000, SDS-PAGE, 1 * 37914, sequence calculation | Mentha x piperita |
43000 | - |
1 x * 43000, SDS-PAGE, 1 * 37914, sequence calculation | Mentha x piperita |
45000 | - |
gel filtration | Mentha x piperita |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (+)-pulegone + 2 NADPH + 2 H+ | Mentha x piperita | pathways of monoterpene biosynthesis, overview | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mentha x piperita | - |
cv. Black Mitcham | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3), native enzyme by oil gland secretory cell isolation procedure, two steps of anion exchange chromatography, hydroxyapatite chromatography, and beta-NADPH affinity chromatography, the activity is rapidly lost during purification on each matrix, 10fold purification accompanied by loss of over 98% of starting activity | Mentha x piperita |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | - |
Mentha x piperita | - |
leaf | - |
Mentha x piperita | - |
secretory cell | - |
Mentha x piperita | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (+)-pulegone + 2 NADPH + 2 H+ | pathways of monoterpene biosynthesis, overview | Mentha x piperita | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? | |
2 (+)-pulegone + 2 NADPH + 2 H+ | the reductase catalyzes the reduction of the 4(8)-double bond of (+)-pulegone to produce both (-)-menthone and (+)-isomenthone in a 70:30 ratio, product analysis by GC-MS, stereospecific reductase | Mentha x piperita | (-)-menthone + (+)-isomenthone + 2 NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 x * 43000, SDS-PAGE, 1 * 37914, sequence calculation | Mentha x piperita |
Synonyms | Comment | Organism |
---|---|---|
More | (+)-pulegone reductase is a member of the medium-chain dehydrogenase/reductase superfamily | Mentha x piperita |
pulegone reductase | - |
Mentha x piperita |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
NADPH | pH 5.0, recombinant enzyme | Mentha x piperita | |
1.8 | - |
(+)-pulegone | pH 5.0, recombinant enzyme | Mentha x piperita |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Mentha x piperita |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | 70% of maximal activity within this range | Mentha x piperita |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Mentha x piperita |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mentha x piperita | about, isoelectric focusing | - |
5.2 |