BRENDA - Enzyme Database show
show all sequences of 1.3.1.81

Organization of monoterpene biosynthesis in Mentha. Immunocytochemical localizations of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase

Turner, G.W.; Croteau, R.; Plant Physiol. 136, 4215-4227 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli strain BL21(DE3)
Mentha x piperita
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Mentha x piperita
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
x * 38000
Mentha x piperita
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 (+)-pulegone + 2 NADPH + 2 H+
Mentha x piperita
the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mentha x piperita
-
cv Black Mitcham
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography followed by SDS-PAGE and gel excision
Mentha x piperita
Source Tissue
Source Tissue
Commentary
Organism
Textmining
secretory trichome
secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development
Mentha x piperita
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 (+)-pulegone + 2 NADPH + 2 H+
the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview
660251
Mentha x piperita
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
-
?
2 (+)-pulegone + 2 NADPH + 2 H+
the reductase catalyzes the reduction of the 4(8)-double bond of (+)-pulegone to produce both (-)-menthone and (+)-isomenthone in a 70:30 ratio
660251
Mentha x piperita
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 38000
Mentha x piperita
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Mentha x piperita
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli strain BL21(DE3)
Mentha x piperita
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Mentha x piperita
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Mentha x piperita
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
x * 38000
Mentha x piperita
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 (+)-pulegone + 2 NADPH + 2 H+
Mentha x piperita
the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography followed by SDS-PAGE and gel excision
Mentha x piperita
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
secretory trichome
secretory cells of peltate glandular trichomes with abundant labeling corresponding to the secretory phase of gland development
Mentha x piperita
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 (+)-pulegone + 2 NADPH + 2 H+
the enzyme is required for catalyzation of the reduction of the DELTA4,8 double bond to produce menthone on route to menthol, monoterpene biosynthetic pathways in peppermint, overview
660251
Mentha x piperita
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
-
?
2 (+)-pulegone + 2 NADPH + 2 H+
the reductase catalyzes the reduction of the 4(8)-double bond of (+)-pulegone to produce both (-)-menthone and (+)-isomenthone in a 70:30 ratio
660251
Mentha x piperita
(-)-menthone + (+)-isomenthone + 2 NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38000
Mentha x piperita
Other publictions for EC 1.3.1.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746411
Li
MAPK-mediated regulation of g ...
Mentha x piperita
Protoplasma
253
1541-1556
2016
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1
-
3
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2
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1
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1
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1
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1
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2
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-
1
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-
-
-
-
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-
2
2
-
-
-
712853
Dolzhenko
UV-B modulates the interplay b ...
Mentha x piperita
J. Photochem. Photobiol. B Biol.
100
67-75
2010
-
-
1
-
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1
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2
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1
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1
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2
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1
2
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1
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1
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1
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1
1
1
1
-
-
713312
Rios-Estepa
Mathematical modeling-guided e ...
Mentha x piperita
Plant Physiol.
152
2105-2119
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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3
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2
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2
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2
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1
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3
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2
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2
2
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725777
Dolzhenko
UV-B modulates the interplay b ...
Mentha x piperita
J. Photochem. Photobiol. B
100
67-75
2010
-
-
-
-
-
-
1
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-
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2
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1
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1
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1
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1
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1
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689800
Rios-Estepa
A systems biology approach ide ...
Mentha x piperita
Proc. Natl. Acad. Sci. USA
105
2818-2823
2008
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-
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1
2
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2
-
2
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1
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2
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3
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1
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1
1
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1
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1
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1
1
1
2
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2
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1
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2
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3
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1
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670589
Ringer
Monoterpene metabolism. Clonin ...
Mentha spicata, Mentha x piperita
Plant Physiol.
137
863-872
2005
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2
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2
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4
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2
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2
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2
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4
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-
660251
Turner
Organization of monoterpene bi ...
Mentha x piperita
Plant Physiol.
136
4215-4227
2004
-
-
1
-
-
-
-
-
1
-
1
1
-
4
-
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1
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1
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2
1
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-
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1
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1
1
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1
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1
1
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1
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1
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2
1
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684664
Ringer
Monoterpene double-bond reduct ...
Mentha x piperita
Arch. Biochem. Biophys.
418
80-92
2003
-
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1
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1
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2
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3
1
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3
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1
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3
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2
1
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2
1
1
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1
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1
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1
1
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1
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2
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3
1
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1
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3
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2
1
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2
1
1
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1
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689735
Mahmoud
Menthofuran regulates essentia ...
Mentha x piperita
Proc. Natl. Acad. Sci. USA
100
14481-14486
2003
-
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-
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1
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1
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2
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3
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1
1
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3
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1
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1
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1
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1
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2
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1
1
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3
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