Any feedback?
Literature summary for 1.3.1.8 extracted from
- Purification of NADPH-dependent enoyl-CoA reductase involved in the malonyl-CoA dependent fatty acid elongation system of Mycobacterium smegmatis (1984), J. Biochem., 96, 841-848.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Isonicotinamide | - |
Mycolicibacterium smegmatis |  |
| monoiodoacetate | - |
Mycolicibacterium smegmatis | |
| N-ethylmaleimide | - |
Mycolicibacterium smegmatis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
(2E)-eicosenoyl-CoA | - |
Mycolicibacterium smegmatis | |
| 0.1 | - |
2-trans-hexadecenoyl-CoA | - |
Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | 60000 | gel filtration | Mycolicibacterium smegmatis |
| 32000 | - |
? * 32000, SDS-PAGE, monomer or dimer | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mycolicibacterium smegmatis | involvement in malonyl-CoA fatty acid elongation system II | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.25 | - |
- |
Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-trans-hexadecenoyl-CoA + NADPH | - |
Mycolicibacterium smegmatis | hexadecanoyl CoA + NADP+ | - |
? | |
| crotonyl-CoA + NAD(P)H | - |
Mycolicibacterium smegmatis | butanoyl-CoA + NADP+ | - |
? | |
| additional information | very slow reduction of substrate with chain length C4, C10, C12 | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | FMN is essential for the reaction, tightly bound to apoprotein | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | involvement in malonyl-CoA fatty acid elongation system II | Mycolicibacterium smegmatis | ? | - |
? | |
| trans-2-eicosenoyl-CoA + NADPH | - |
Mycolicibacterium smegmatis | eicosanoyl-CoA + NADP+ | i.e. arachidoyl-CoA | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | ? * 32000, SDS-PAGE, monomer or dimer | Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Mycolicibacterium smegmatis |
| 7 | - |
assay at | Mycolicibacterium smegmatis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | 1 mol per mol of protein, prosthetic group is essentiell for the reaction | Mycolicibacterium smegmatis | |
| NADPH | inactive with NADH | Mycolicibacterium smegmatis | |
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