Purification of NADPH-dependent enoyl-CoA reductase involved in the malonyl-CoA dependent fatty acid elongation system of Mycobacterium smegmatis

Kikuchi, S.; Kusaka, T.; J. Biochem. 96, 841-848 (1984)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

Isonicotinamide

-

Mycolicibacterium smegmatis

monoiodoacetate

-

Mycolicibacterium smegmatis

N-ethylmaleimide

-

Mycolicibacterium smegmatis

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.083

-

(2E)-eicosenoyl-CoA

-

Mycolicibacterium smegmatis

0.1

-

2-trans-hexadecenoyl-CoA

-

Mycolicibacterium smegmatis

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

additional information

Mycolicibacterium smegmatis

involvement in malonyl-CoA fatty acid elongation system II

?

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2-trans-hexadecenoyl-CoA + NADPH

-

390849

Mycolicibacterium smegmatis

hexadecanoyl CoA + NADP+

-

-

-

?

crotonyl-CoA + NAD(P)H

-

390849

Mycolicibacterium smegmatis

butanoyl-CoA + NADP+

-

-

-

?

additional information

very slow reduction of substrate with chain length C4, C10, C12

390849

Mycolicibacterium smegmatis

?

-

-

-

?

additional information

FMN is essential for the reaction, tightly bound to apoprotein

390849

Mycolicibacterium smegmatis

?

-

-

-

?

additional information

involvement in malonyl-CoA fatty acid elongation system II

390849

Mycolicibacterium smegmatis

?

-

-

-

?

trans-2-eicosenoyl-CoA + NADPH

-

390849

Mycolicibacterium smegmatis

eicosanoyl-CoA + NADP+

i.e. arachidoyl-CoA

390849

Mycolicibacterium smegmatis

?

Cofactor

Commentary

Organism

Structure

FMN

1 mol per mol of protein, prosthetic group is essentiell for the reaction

Mycolicibacterium smegmatis

NADPH

inactive with NADH

Mycolicibacterium smegmatis

Cofactor

Commentary

Organism

Structure

FMN

1 mol per mol of protein, prosthetic group is essentiell for the reaction

Mycolicibacterium smegmatis

NADPH

inactive with NADH

Mycolicibacterium smegmatis

Inhibitors

Commentary

Organism

Structure

Isonicotinamide

-

Mycolicibacterium smegmatis

monoiodoacetate

-

Mycolicibacterium smegmatis

N-ethylmaleimide

-

Mycolicibacterium smegmatis

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.083

-

(2E)-eicosenoyl-CoA

-

Mycolicibacterium smegmatis

0.1

-

2-trans-hexadecenoyl-CoA

-

Mycolicibacterium smegmatis

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

additional information

Mycolicibacterium smegmatis

involvement in malonyl-CoA fatty acid elongation system II

?

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2-trans-hexadecenoyl-CoA + NADPH

-

390849

Mycolicibacterium smegmatis

hexadecanoyl CoA + NADP+

-

-

-

?

crotonyl-CoA + NAD(P)H

-

390849

Mycolicibacterium smegmatis

butanoyl-CoA + NADP+

-

-

-

?

additional information

very slow reduction of substrate with chain length C4, C10, C12

390849

Mycolicibacterium smegmatis

?

-

-

-

?

additional information

FMN is essential for the reaction, tightly bound to apoprotein

390849

Mycolicibacterium smegmatis

?

-

-

-

?

additional information

involvement in malonyl-CoA fatty acid elongation system II

390849

Mycolicibacterium smegmatis

?

-

-

-

?

trans-2-eicosenoyl-CoA + NADPH

-

390849

Mycolicibacterium smegmatis

eicosanoyl-CoA + NADP+

i.e. arachidoyl-CoA

390849

Mycolicibacterium smegmatis

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

712769

Sugishima

Crystal structures of the subs ...

Arabidopsis thaliana

J. Mol. Biol.

402

879-891

2010

-

-

1

1

1

-

-

-

-

-

-

1

-

1

-

-

1

-

-

-

-

-

2

1

1

-

-

-

-

-

-

-

2

-

-

-

-

-

1

2

1

1

-

-

-

-

-

-

-

-

1

-

-

-

1

-

-

-

-

2

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

685980

Rennison

Enhanced acyl-CoA dehydrogenas ...

Rattus norvegicus

Cardiovasc. Res.

79

331-340

2008

-

-

-

-

-

-

-

-

1

-

-

1

-

1

-

-

-

-

-

1

-

-

3

-

2

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

1

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

689745

Erb

Synthesis of C5-dicarboxylic a ...

Luteovulum sphaeroides

Proc. Natl. Acad. Sci. USA

104

10631-10636

2007

-

-

1

-

-

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

2

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

673619

Park

Silencing of NbECR encoding a ...

Nicotiana benthamiana

FEBS Lett.

579

4459-4464

2005

-

-

-

-

-

-

-

-

2

-

1

1

-

4

-

-

-

-

-

6

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

1

1

-

-

-

-

-

6

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

656737

Li

Crotonyl-coenzyme A reductase ...

Streptomyces collinus

Microbiology

150

3463-3472

2004

-

2

1

-

-

-

-

-

-

-

-

1

-

6

-

-

-

1

-

1

1

-

2

-

2

-

-

-

-

-

-

-

-

-

-

-

-

2

1

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390872

Sargueil

High metabolism and subsequent ...

Mus musculus

Neurosci. Lett.

273

29-32

1999

-

-

-

-

-

-

-

-

1

-

-

2

-

2

-

-

-

-

-

1

4

-

3

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

2

-

-

-

-

-

1

4

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390873

Lessire

Study of the 3-hydroxy-eicosan ...

Allium ampeloprasum

Plant Physiol.

119

1009-1015

1999

-

-

-

-

-

-

-

-

1

-

-

1

-

3

-

-

-

-

-

1

1

-

2

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

1

1

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390869

Mukherjee

Inactivation of enoyl-CoA redu ...

Columba sp.

J. Enzyme Inhib.

13

217-228

1998

-

-

-

-

-

-

1

-

-

-

-

-

-

3

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390868

Mukherjee

Evidence for the essential his ...

Columba sp.

J. Enzyme Inhib.

11

209-216

1997

-

-

-

-

-

-

1

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390759

Laguna

Action of Ebselen on rat hepat ...

Rattus norvegicus

Arch. Biochem. Biophys.

269

272-283

1989

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

1

-

-

2

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

1

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390758

Nagi

Dual action of 2-decynoyl coen ...

Rattus norvegicus

Arch. Biochem. Biophys.

267

1-12

1988

-

-

-

-

-

-

1

-

1

-

-

-

-

1

-

-

-

-

-

1

-

-

2

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

1

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390757

Nagi

Site of inhibition of rat live ...

Rattus norvegicus

J. Biol. Chem.

261

13598-13605

1986

-

-

-

-

-

-

1

-

1

-

-

-

-

1

-

-

-

-

-

1

-

-

1

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390753

Prasad

Solubilization and purificatio ...

Rattus norvegicus

Arch. Biochem. Biophys.

237

535-544

1985

-

-

-

-

-

-

1

4

1

-

-

-

-

3

-

-

1

-

-

1

1

-

8

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

4

1

-

-

-

-

-

-

1

-

1

1

-

8

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390870

Cvetanovic

Purification and characterizat ...

Bos taurus

Biochem. J.

227

49-56

1985

-

-

-

-

-

-

5

-

2

-

4

-

-

6

-

-

1

-

-

5

5

-

8

1

1

1

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

5

-

-

2

-

4

-

-

-

-

1

-

5

5

-

8

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

390810

Inui

Fatty acid synthesis in mitoch ...

Euglena gracilis

Eur. J. Biochem.

142

121-126

1984

-

-

-

-

-

-

-

-

3

-

-

1

-

5

-

-

-

-

-

-

-

-

9

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

-

-

-

3

-

-

1

-

-

-

-

-

-

-

-

9

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390849

Kikuchi

Purification of NADPH-dependen ...

Mycolicibacterium smegmatis

J. Biochem.

96

841-848

1984

-

-

-

-

-

-

3

2

-

-

2

1

-

10

-

-

1

-

-

-

1

-

6

1

-

1

-

-

-

2

-

-

2

-

-

-

-

-

-

2

-

-

-

-

3

-

2

-

-

2

1

-

-

-

1

-

-

1

-

6

1

1

-

-

-

2

-

-

-

-

-

-

-

-

-

390754

Nagi

Biochemical properties of shor ...

Rattus norvegicus

Arch. Biochem. Biophys.

226

50-64

1983

-

-

-

-

-

-

-

-

1

-

-

-

-

2

-

-

-

-

-

1

-

-

3

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390755

Prasad

Kinetic evidence for two separ ...

Rattus norvegicus

Biochem. Biophys. Res. Commun.

113

659-665

1983

-

-

-

-

-

-

-

-

1

-

-

-

-

2

-

-

-

-

-

1

-

-

5

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

5

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390756

Cinti

Evidence for a second microsom ...

Rattus norvegicus

J. Biol. Chem.

257

14333-14340

1982

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

1

-

-

3

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390752

Bernert

The isolation of acyl-CoA deri ...

Rattus norvegicus

Biochim. Biophys. Acta

573

436-442

1979

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

-

-

-

-

1

-

-

1

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390850

Strom

-

Activation and inhibition of c ...

Bos taurus

J. Biol. Chem.

254

8159-8162

1979

1

-

-

-

-

-

9

-

-

-

-

-

-

1

-

-

-

-

-

1

-

1

1

-

-

-

-

-

-

1

1

-

1

4

-

-

1

-

-

1

-

-

-

-

9

4

-

-

-

-

-

-

-

-

-

-

1

-

1

1

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

390851

Strom

Enoyl coenzyme A reduction by ...

Bos taurus

J. Biol. Chem.

254

8153-8158

1979

-

-

-

-

-

1

2

-

-

-

-

-

-

3

1

-

1

-

-

1

1

1

8

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

1

-

2

-

-

-

-

-

-

-

1

-

1

-

1

1

1

8

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390751

Bernert

An analysis of partial reactio ...

Rattus norvegicus

J. Biol. Chem.

252

6736-6744

1977

1

-

-

-

-

-

2

-

1

-

-

-

-

1

-

-

-

-

-

1

-

-

6

-

1

-

-

-

-

1

-

-

1

-

-

-

1

-

-

1

-

-

-

-

2

-

-

1

-

-

-

-

-

-

-

-

1

-

-

6

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

390852

Maitra

Crotonyl coenzyme A reductase ...

Bos taurus

J. Biol. Chem.

249

111-117

1974

1

-

-

-

-

-

1

3

-

-

-

-

-

3

-

-

1

-

-

1

-

-

2

-

-

1

1

-

-

1

-

-

2

-

-

-

1

-

-

2

-

-

-

-

1

-

3

-

-

-

-

-

-

-

1

-

1

-

-

2

-

1

1

-

-

1

-

-

-

-

-

-

-

-

-

390853

Ishidate

Induction of NADPH enoyl-coA r ...

Candida albicans

J. Biochem.

76

1139-1142

1974

-

-

-

-

-

-

-

-

-

-

-

2

-

3

-

-

-

-

-

-

1

-

4

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

1

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390854

Ishidate

Separation and some characteri ...

Candida albicans

Chem. Pharm. Bull.

22

2685-2691

1974

-

-

-

-

-

-

-

6

-

-

2

2

-

1

-

-

1

-

-

-

1

-

4

-

-

-

-

2

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

6

-

-

2

2

-

-

-

1

-

-

1

-

4

-

-

-

2

-

1

-

-

-

-

-

-

-

-

-

390855

Mizugaki

Stereospecific hydrogen transf ...

Rattus norvegicus

J. Biochem.

74

691-696

1973

-

-

-

-

-

-

-

-

1

-

-

1

-

1

-

-

-

-

-

1

-

-

3

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

1

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

390856

Mizugaki

Possible role of NADPH-depende ...

Rattus norvegicus

Biochem. Biophys. Res. Commun.

50

48-53

1973

-

-

-

-

-

-

3

1

1

-

-

1

-

1

-

-

-

-

-

1

-

-

3

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

3

-

1

1

-

-

1

-

-

-

-

-

1

-

-

3

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

390857

Podack

On the mechanism of malonyl-Co ...

Rattus norvegicus

Biochim. Biophys. Acta

280

235-247

1972

-

-

-

-

-

-

-

2

1

-

-

1

-

1

-

-

-

-

-

1

1

-

3

-

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

2

1

-

-

1

-

-

-

-

-

1

1

-

3

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

390858

Seubert

On the mechanism of malonyl-Co ...

Rattus norvegicus

Biochim. Biophys. Acta

164

498-517

1968

-

-

-

-

-

-

-

-

1

-

-

2

-

1

-

-

1

-

-

1

1

1

4

-

2

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

1

-

-

2

-

-

-

1

-

1

1

1

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-