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Literature summary for 1.3.1.77 extracted from

  • Zhu, Y.; Wang, H.; Peng, Q.; Tang, Y.; Xia, G.; Wu, J.; Xie, D.Y.
    Functional characterization of an anthocyanidin reductase gene from the fibers of upland cotton (Gossypium hirsutum) (2015), Planta, 241, 1075-1089 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene GhANR1, cloned from developing fibers, DNA and amino acid sequence determination and analysis, ectopic expression of GhANR11 in the Arabidopsis thaliana ban mutant, via transformation by Agrobacterium tumefaciens strain EHA 105, allows for the reconstruction of the ANR pathway and proanthocyanidin biosynthesis in the seed coat, recombinant expression of His-Trx-tagged enzyme in Escherichia coli strain BL21(DE3) Gossypium hirsutum

Engineering

Protein Variants Comment Organism
additional information virus-induced gene silencing (VIGS) of GhANR11 in Gossypium hirsutum leads to a significant increase in anthocyanins and a decrease in the proanthocyanidins, (-)-epicatechin, and (-)-catechin in the stems and leaves of VIGS-infected plants. Anthocyanin accumulation in leaves and stems increases in VIGS-infected tissue compared to wild-type, phenotype, overview Gossypium hirsutum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 cyanidin + 4 NADPH + H+ Gossypium hirsutum
-
(-)-catechin + (-)-epicatechin + 4 NADP+ ? ?
2 delphinidin + 4 NADPH + H+ Gossypium hirsutum
-
(-)-gallocatechin + (-)-epigallocatechin + 4 NADP+ ? ?
2 pelargonidin + 4 NADPH + H+ Gossypium hirsutum
-
(-)-afzelechin + (-)-epiafzelechin + 4 NADP+ ? ?

Organism

Organism UniProt Comment Textmining
Gossypium hirsutum A2IBG2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-Trx-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Gossypium hirsutum

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Gossypium hirsutum
-
seed
-
Gossypium hirsutum
-
leaf
-
Gossypium hirsutum
-
stem
-
Gossypium hirsutum
-
additional information analysis of GhANR1 expression in developing fibers of Gossypium hirsutum and transgenic Arabidosis thaliana seedlings, overview. Proanthocyanidin contents in different tissues of Gossypium hirsutum, TLC and HPLC determination, overview Gossypium hirsutum
-
fiber
-
Gossypium hirsutum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 cyanidin + 4 NADPH + H+
-
Gossypium hirsutum (-)-catechin + (-)-epicatechin + 4 NADP+ ? ?
2 delphinidin + 4 NADPH + H+
-
Gossypium hirsutum (-)-gallocatechin + (-)-epigallocatechin + 4 NADP+ ? ?
2 pelargonidin + 4 NADPH + H+
-
Gossypium hirsutum (-)-afzelechin + (-)-epiafzelechin + 4 NADP+ ? ?

Synonyms

Synonyms Comment Organism
ANR
-
Gossypium hirsutum
anthocyanidin reductase
-
Gossypium hirsutum
GhANR1
-
Gossypium hirsutum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
assay at Gossypium hirsutum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
assay at Gossypium hirsutum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Gossypium hirsutum

General Information

General Information Comment Organism
physiological function the anthocyanidin reductase (ANR) pathway is involved in the biosynthesis of proanthocyanidins in upland cotton Gossypium hirsutum