Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.77 extracted from

  • Kumar, V.; Yadav, S.K.
    Pyramiding of tea dihydroflavonol reductase and anthocyanidin reductase increases flavan-3-ols and improves protective ability under stress conditions in tobacco (2017), 3 Biotech, 7, 177 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.77

Cloned(Commentary)

Cloned (Comment) Organism
gene ANR1, co-overexpression with leucoanthocyanidin reductase (LAR) in Nicotiana tabacum cv. Xanthi by Agrobacterium tumefaciens-mediated transformation, semiquantitative expression analysis Camellia sinensis
gene ANR2, co-overexpression with leucoanthocyanidin reductase (LAR) in Nicotiana tabacum cv. Xanthi by Agrobacterium tumefaciens-mediated transformation, semiquantitative expression analysis Camellia sinensis

Protein Variants

Protein Variants Comment Organism
additional information generation of pyramided transgenic lines overexpressing CsDFR and CsANR is implemented for evaluation purpose in relation to flavan-3-ol accumulation and for analyzing their combinatorial influence on the improvement of overall antioxidant potential, no morphological difference is observed between the CsDFR/CsANR overexpressing transgenic plants generated by reciprocal crosses with each other. No significant changes in growth pattern are observed in pyramided transgenic tobacco lines as compared to control tobacco plants. Both reciprocal crosses do not show significant change in plant height, stem diameter, and number of leaves as compared to control tobacco plants, which are measured at the flowering time, but the pyramided transgenic lines show a higher number of fruits/capsules per plant, increased seed yield per plant, and weight of seed (grams) compared to control tobacco plants. Increased transcript expression of flavan-3-ol/anthocyanin biosynthetic pathway genes in pyramided transgenic tobacco plants occurs. The transcript expression of NtAN2 is upregulated by 106-154% in selected lines as compared to control tobacco plant. Improved antioxidant potential under the influence of CsDFR and CsANR overexpression in pyramided transgenic tobacco plants. Phenotype, overview Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ Camellia sinensis
-
 an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+
-
 ?
anthocyanidin + 2 NADPH + H+ Camellia sinensis
-
 epicatechin + 2 NADP+ ? ?

Organism

Organism UniProt Comment Textmining
Camellia sinensis F8V3W3
-
-
Camellia sinensis H9LBK6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Camellia sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a (2R,3R)-flavan-3-ol + 2 NAD(P)+
-
 Camellia sinensis an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+
-
 ?
anthocyanidin + 2 NADPH + H+
-
 Camellia sinensis epicatechin + 2 NADP+ ? ?

Synonyms

Synonyms Comment Organism
ANR
-
 Camellia sinensis
ANR1
-
 Camellia sinensis
ANR2
-
 Camellia sinensis
anthocyanidin reductase
-
 Camellia sinensis
anthocyanidin reductase 1
-
 Camellia sinensis
anthocyanidin reductase 2
-
 Camellia sinensis
CsANR
-
 Camellia sinensis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Camellia sinensis
NADPH
-
 Camellia sinensis

General Information

General Information Comment Organism
metabolism the enzyme is involved in the flavan-3-ol/anthocyanin biosynthetic pathway. Leucoanthocyanidin reductase (LAR, EC 1.17.1.3) and anthocyanidin reductase (ANR) catalyze the formation of catechins and epicatechins from leucoanthocyanidins and anthocyanidins, respectively, overview Camellia sinensis