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Literature summary for 1.3.1.77 extracted from

  • Zhu, Y.; Peng, Q.; Li, K.; Xie, D.
    Molecular cloning and functional characterization of the anthocyanidin reductase gene from Vitis bellula (2014), Planta, 240, 381-398.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vitis bellula

Inhibitors

Inhibitors Comment Organism Structure
sodium chloride 500 mm, almost complete loss of activity Vitis bellula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.131
-
delphinidin pH 4.0, 40°C Vitis bellula
0.135
-
pelargonidin pH 4.0, 40°C Vitis bellula
0.176
-
cyanidin pH 4.0, 40°C Vitis bellula
0.193
-
NADPH pH 4.0, 40°C Vitis bellula
0.817
-
NADH pH 4.0, 40°C Vitis bellula

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Vitis bellula 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
x * 56000, SDS-PAGE Vitis bellula

Organism

Organism UniProt Comment Textmining
Vitis bellula H9TZS7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanidin + 2 NADPH + 2 H+
-
Vitis bellula (-)-catechin + (-)-epicatechin + 2 NADP+
-
?
delphinidin + 2 NADH + 2 H+
-
Vitis bellula (-)-epigallocatechin + (-)-gallocatechin + 2 NAD+
-
?
additional information ANR converts cyanidin, delphinidin, and pelargonidin to their corresponding flavan-3-ols. Enzymatic products include 2S,3R-trans, 2R,3R-cis and probably 2S,3S-cis-flavan-3-ol isomers Vitis bellula ?
-
?
pelargonidin + 2 NADPH + 2 H+
-
Vitis bellula (-)-epiafzelechin + (-)-afzelechin + 2 NADP+
-
?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Vitis bellula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Vitis bellula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.66
-
cyanidin pH 4.0, 40°C Vitis bellula
2
-
pelargonidin pH 4.0, 40°C Vitis bellula
2.01
-
delphinidin pH 4.0, 40°C Vitis bellula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Vitis bellula

Cofactor

Cofactor Comment Organism Structure
NADH NADPH is preferred over NADH Vitis bellula
NADPH NADPH is preferred over NADH Vitis bellula

General Information

General Information Comment Organism
physiological function overexpression of ANR in banyuls mutants reconstructs the biosynthetic pathway of proanthocyanidins in the seed coat Vitis bellula

KCat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.48
-
cyanidin pH 4.0, 40°C Vitis bellula
14.9
-
pelargonidin pH 4.0, 40°C Vitis bellula
16.4
-
delphinidin pH 4.0, 40°C Vitis bellula